International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai, China.
Biofouling. 2023 Mar-Apr;39(4):359-370. doi: 10.1080/08927014.2023.2215707. Epub 2023 Jun 9.
Despite the importance of outer membrane vesicles (OMVs) in benthic animal settlement, the underlying molecular mechanism remains elusive. Here, the impact of OMVs and OMVs synthesis-related gene in plantigrade settlement was tested. The OMVs were extracted from through density gradient centrifugation, and a knockout strain, achieved by homologous recombination, was utilized for the investigation. Our results demonstrated that OMVs could significantly enhance plantigrades settlement. Deleting the resulted in downregulation of c-di-GMP, accompanied by a reduction of OMV production, a decline in bacterial motility and increasing biofilm-forming ability. Enzyme treatment resulted in a 61.11% reduction in OMV-inducing activity and a 94.87% reduction in LPS content. Thus, OMVs regulate mussel settlement LPS, and c-di-GMP is responsible for the OMV-inducing capacity. These findings provide new insights into the interactions between bacteria and mussels.
尽管外膜囊泡（OMVs）在底栖动物定殖中具有重要作用，但其中的潜在分子机制仍难以捉摸。在这里，我们测试了 OMVs 和与 OMVs 合成相关基因对植物状定殖的影响。通过密度梯度离心从贻贝中提取 OMVs，并利用同源重组获得了一株缺失菌株用于研究。我们的结果表明，OMVs 可以显著增强贻贝的定殖。敲除 基因导致 c-di-GMP 的下调，同时伴随着 OMV 产量的减少、细菌运动性的降低和生物膜形成能力的增加。酶处理导致 OMV 诱导活性降低 61.11%，LPS 含量降低 94.87%。因此，OMVs 通过 LPS 调节贻贝定殖，而 c-di-GMP 则负责 OMV 的诱导能力。这些发现为细菌和贻贝之间的相互作用提供了新的见解。
