College of Food and Biological Engineering (Liquor College), Henan University of Animal Husbandry and Economy, Zhengzhou, China.
Henan Liquor Style Engineering Technology Research Center, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
J Sci Food Agric. 2023 Nov;103(14):6849-6860. doi: 10.1002/jsfa.12773. Epub 2023 Jun 21.
There are few reports on the breeding of high-yielding tetramethylpyrazine (TTMP) strains in strong-flavor Daqu. In addition, studies on the mechanism of TTMP production in strains are mostly based on common physiological and biochemical indicators, and there is no report on RNA level. Therefore, in this study, a strain with high production of TTMP was screened out from strong-flavor liquor, and transcriptome sequencing analysis was performed to analyze its key metabolic pathways and key genes, and to infer the mechanism of TTMP production in the strain.
In this study, a strain with a high yield of tetramethylpyrazine (TTMP) was screened out, and the yield was 29.83 μg mL . The identified strain was Bacillus velezensis, which could increase the content of TTMP in liquor by about 88%. After transcriptome sequencing, a total of 1851 differentially expressed genes were screened, including 1055 up-regulated genes and 796 down-regulated genes. Three pathways related to the production of TTMP were identified by gene ontology (GO) annotation and COG annotation, including carbohydrate metabolism, cell movement and amino acid metabolism. The key genes of TTMP were analyzed, and the factors that might regulate the production of TTMP, such as the transfer of uracil phosphate ribose and glycosyltransferase, were obtained.
A strain of B. velezensis with high TTMP production was screened and identified in strong-flavor Daqu for the first time. The yield of TTMP was 29.83 μg mL , which increased the TTMP content in liquor by 88%. The key metabolic pathways of TTMP production in the strain were obtained: carbohydrate metabolism, cell movement and amino acid metabolism, and the key regulatory genes of each pathway were found, which complemented the gap in gene level in the production regulation of the strain, and provided a theoretical basis for the subsequent study of TTMP in liquor. © 2023 Society of Chemical Industry.
在浓香型大曲中高产四甲基吡嗪（TTMP）菌株的选育报道较少。此外，对菌株 TTMP 产生机制的研究大多基于常见的生理生化指标，尚无 RNA 水平的报道。因此，本研究从浓香型白酒中筛选出一株 TTMP 高产菌株，进行转录组测序分析，分析其关键代谢途径和关键基因，推断菌株中 TTMP 产生的机制。
本研究筛选出一株 TTMP 产量较高的菌株，产量为 29.83μg·mL-1。鉴定出的菌株为解淀粉芽孢杆菌，可使白酒中 TTMP 的含量提高约 88%。经转录组测序，共筛选出 1851 个差异表达基因，其中上调基因 1055 个，下调基因 796 个。通过基因本体（GO）注释和 COG 注释，鉴定出与 TTMP 产生相关的 3 条途径，包括碳水化合物代谢、细胞运动和氨基酸代谢。分析 TTMP 的关键基因，获得可能调节 TTMP 产生的因素，如尿苷磷酸核糖转移酶和糖基转移酶。
首次从浓香型大曲中筛选鉴定出一株高产 TTMP 的解淀粉芽孢杆菌，其 TTMP 产量为 29.83μg·mL-1，使白酒中 TTMP 的含量提高了 88%。获得了菌株中 TTMP 产生的关键代谢途径：碳水化合物代谢、细胞运动和氨基酸代谢，并找到了各途径的关键调控基因，补充了该菌株在生产调控基因水平上的空白，为后续白酒中 TTMP 的研究提供了理论依据。© 2023 化学工业协会。
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