芽孢杆菌属TTMP20的转录组测序及BDH基因对川芎嗪合成影响的分析
Transcriptome Sequencing of Bacillus sp. TTMP20 and Analysis of the Effect of BDH Gene on Tetramethylpyrazine Synthesis.
作者信息
Liang Shiyu, Ling Mengxiang, Li Lihua, Gan Shanling, Shang Changhua
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
出版信息
Curr Microbiol. 2024 Dec 9;82(1):33. doi: 10.1007/s00284-024-03990-5.
Bacillus sp. TTMP20 has high tetramethylpyrazine (TTMP) yield. However, the mechanism of TTMP production in this strain is unclear at present, which limits the modification for strain TTMP20. In this study, key metabolic pathways related to TTMP synthesis were identified, which included glycolytic pathway, tricarboxylic acid cycle and nitrogen metabolism pathway. Moreover, transgenic Bacillus sp. TTMP20 with recombinant vector PHY-300PLK-BDH was constructed. 2,3-butanediol dehydrogenase gene (BDH) overexpression considerably reduced TTMP biosynthesis. This study will supply new insight into the regulation of TTMP biosynthesis at key enzyme gene level.
芽孢杆菌属TTMP20具有较高的四甲基吡嗪（TTMP）产量。然而，目前该菌株中TTMP的产生机制尚不清楚，这限制了对菌株TTMP20的改造。在本研究中，确定了与TTMP合成相关的关键代谢途径，包括糖酵解途径、三羧酸循环和氮代谢途径。此外，构建了携带重组载体PHY-300PLK-BDH的转基因芽孢杆菌属TTMP20。2,3-丁二醇脱氢酶基因（BDH）的过表达显著降低了TTMP的生物合成。本研究将为在关键酶基因水平上调控TTMP生物合成提供新的见解。
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