血管紧张素II构象的质子磁共振研究：含肌氨酸7的类似物中的顺反异构现象

Proton magnetic resonance studies of angiotensin II conformation: cis-trans isomerism in sarcosine7-containing analogs.

作者信息

Matsoukas J M, Moore G J

出版信息

Arch Biochem Biophys. 1986 Jul;248(1):419-23. doi: 10.1016/0003-9861(86)90438-8.

DOI:10.1016/0003-9861(86)90438-8

Abstract

1H-NMR spectra for the angiotensin agonist sarcosine-(Sar)Arg-Val-Tyr-Ile-His-Sar-Phe [( Sar1,Sar7]Ang II) and the antagonist Sar-Arg-Val-Tyr-Ile-His-Sar-Ile in dimethylsulfoxide-d6 were examined at 400 MHz. Splitting of the resonances for Tyr, His, and Sar protons revealed that the His6-Sar7 peptide bond existed in both cis and trans forms, with one isomer predominating in the ratio 5:1 in both peptides. Comparison of the chemical shifts for the His6 and Phe8 ring protons in these peptides suggested a His/Phe stacking interaction in [Sar1,Sar7]Ang II which is important for agonist activity.

摘要

在400兆赫下检测了血管紧张素激动剂肌氨酸 -（Sar）精氨酸 - 缬氨酸 - 酪氨酸 - 异亮氨酸 - 组氨酸 - 肌氨酸 - 苯丙氨酸[（Sar1，Sar7）血管紧张素II]和拮抗剂肌氨酸 - 精氨酸 - 缬氨酸 - 酪氨酸 - 异亮氨酸 - 组氨酸 - 肌氨酸 - 异亮氨酸在氘代二甲亚砜中的1H - NMR谱。酪氨酸、组氨酸和肌氨酸质子共振峰的分裂表明，组氨酸6 - 肌氨酸7肽键以顺式和反式两种形式存在，在两种肽中，一种异构体占主导，比例为5:1。这些肽中组氨酸6和苯丙氨酸8环质子化学位移的比较表明，（Sar1，Sar7）血管紧张素II中存在组氨酸/苯丙氨酸堆积相互作用，这对激动剂活性很重要。

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Arch Biochem Biophys. 1986 Jul;248(1):419-23. doi: 10.1016/0003-9861(86)90438-8.

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