Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, PR China; Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
J Hazard Mater. 2023 Sep 5;457:131725. doi: 10.1016/j.jhazmat.2023.131725. Epub 2023 May 27.
An important public concern worldwide is soil pollution caused by organophosphorus pesticides and their primary metabolites. To protect the public's health, screening these pollutants on-site and determining their soil bioavailability is important, but doing so is still challenging. This work improved the already-existing organophosphorus pesticide hydrolase (mpd) and transcriptional activator (pobR), and it first designed and constructed a novel biosensor (Escherichia coli BL21/pNP-LacZ) that can precisely detect methyl parathion (MP) and its primary metabolite p-nitrophenol with low background value. To create a paper strip biosensor, E. coli BL21/pNP-LacZ was fixed to filter paper using bio-gel alginate and sensitizer polymyxin B. According to the calibrations of the paper strip biosensor for soil extracts and standard curve, the color intensity of the paper strip biosensor collected by the mobile app may be used to compute the concentration of MP and p-nitrophenol. This method's detection limits were 5.41 µg/kg for p-nitrophenol and 9.57 µg/kg for MP. The detection of p-nitrophenol and MP in laboratory and field soil samples confirmed this procedure. Paper strip biosensor on-site allows for the semi-quantitative measurement of p-nitrophenol and MP levels in soils in a simple, inexpensive, and portable method.
全世界都非常关注有机磷农药及其初级代谢物造成的土壤污染问题。为了保护公众健康，现场筛选这些污染物并确定其土壤生物有效性非常重要，但这仍然具有挑战性。本工作改进了现有的有机磷农药水解酶（mpd）和转录激活因子（pobR），并首次设计和构建了一种新型生物传感器（大肠杆菌 BL21/pNP-LacZ），该传感器可以精确检测甲基对硫磷（MP）及其初级代谢物对硝基苯酚，背景值低。为了制作纸条生物传感器，使用生物凝胶海藻酸钠和敏化剂多粘菌素 B 将大肠杆菌 BL21/pNP-LacZ 固定在滤纸上。根据纸条生物传感器对土壤提取物和标准曲线的校准，移动应用程序收集的纸条生物传感器的颜色强度可用于计算 MP 和对硝基苯酚的浓度。该方法对 p-硝基苯酚的检测限为 5.41µg/kg，对 MP 的检测限为 9.57µg/kg。实验室和田间土壤样品的检测结果证实了该方法的可行性。纸条生物传感器可现场用于以简单、廉价和便携的方式对土壤中的 p-硝基苯酚和 MP 水平进行半定量测量。
