IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy.
Department of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, Italy.
Int J Mol Sci. 2023 May 30;24(11):9486. doi: 10.3390/ijms24119486.
Cannabinoids, natural or synthetic, have antidepressant, anxiolytic, anticonvulsant, and anti-psychotic properties. Cannabidiol (CBD) and delta-9-tetrahydrocannabinol (Δ-THC) are the most studied cannabinoids, but recently, attention has turned towards minor cannabinoids. Delta-8-tetrahydrocannabinol (Δ-THC), an isomer of Δ-THC, is a compound for which, to date, there is no evidence of its role in the modulation of synaptic pathways. The aim of our work was to evaluate the effects of Δ-THC on differentiated SH-SY5Y human neuroblastoma cells. Using next generation sequencing (NGS), we investigated whether Δ-THC could modify the transcriptomic profile of genes involved in synapse functions. Our results showed that Δ-THC upregulates the expression of genes involved in the glutamatergic pathway and inhibits gene expression at cholinergic synapses. Conversely, Δ-THC did not modify the transcriptomic profile of genes involved in the GABAergic and dopaminergic pathways.
大麻素，无论是天然的还是合成的，都具有抗抑郁、抗焦虑、抗惊厥和抗精神病的特性。大麻二酚 (CBD) 和 Δ-9-四氢大麻酚 (Δ-THC) 是研究最多的大麻素，但最近，人们的注意力转向了次要大麻素。Δ-8-四氢大麻酚 (Δ-THC) 是 Δ-THC 的一种异构体，迄今为止，尚无证据表明其在调节突触通路中的作用。我们工作的目的是评估 Δ-THC 对分化的 SH-SY5Y 人神经母细胞瘤细胞的影响。使用下一代测序 (NGS)，我们研究了 Δ-THC 是否可以改变参与突触功能的基因的转录组特征。我们的结果表明，Δ-THC 上调了参与谷氨酸能途径的基因的表达，并抑制了胆碱能突触的基因表达。相反，Δ-THC 并未改变参与 GABA 能和多巴胺能途径的基因的转录组特征。
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