Pharmacology Department, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Pharmacol Biochem Behav. 2010 Jun;95(4):434-42. doi: 10.1016/j.pbb.2010.03.004. Epub 2010 Mar 21.
The antidepressant action of cannabis as well as the interaction between antidepressants and the endocannabinoid system has been reported. This study was conducted to assess the antidepressant-like activity of Delta(9)-THC and other cannabinoids. Cannabinoids were initially evaluated in the mouse tetrad assay to determine doses that do not induce hypothermia or catalepsy. The automated mouse forced swim (FST) and tail suspension (TST) tests were used to determine antidepressant action. At doses lacking hypothermic and cataleptic effects (1.25, 2.5, and 5 mg/kg, i.p.), both Delta(9)-THC and Delta(8)-THC showed a U-shaped dose response with only Delta(9)-THC showing significant antidepressant-like effects at 2.5 mg/kg (p<0.05) in the FST. The cannabinoids cannabigerol (CBG) and cannabinol (CBN) did not produce antidepressant-like actions up to 80 mg/kg in the mouse FST, while cannabichromene (CBC) and cannabidiol (CBD) exhibited significant effect at 20 and 200mg/kg, respectively (p<0.01). The antidepressant-like action of Delta(9)-THC and CBC was further confirmed in the TST. Delta(9)-THC exhibited the same U-shaped dose response with significant antidepressant-like action at 2.5 mg/kg (p<0.05) while CBC resulted in a significant dose-dependent decrease in immobility at 40 and 80 mg/kg doses (p<0.01). Results of this study show that Delta(9)-THC and other cannabinoids exert antidepressant-like actions, and thus may contribute to the overall mood-elevating properties of cannabis.
大麻的抗抑郁作用以及抗抑郁药与内源性大麻素系统之间的相互作用已经有报道。本研究旨在评估Δ9-四氢大麻酚和其他大麻素的抗抑郁样活性。大麻素最初在小鼠四联测试中进行评估，以确定不引起体温过低或僵住的剂量。自动小鼠强迫游泳（FST）和悬尾（TST）测试用于确定抗抑郁作用。在缺乏体温过低和僵住作用的剂量下（1.25、2.5 和 5mg/kg，ip），Δ9-THC 和 Δ8-THC 均表现出 U 形剂量反应，只有 Δ9-THC 在 FST 中在 2.5mg/kg 时表现出显著的抗抑郁样作用（p<0.05）。大麻素大麻萜酚（CBG）和大麻酚（CBN）在小鼠 FST 中高达 80mg/kg 时没有产生抗抑郁样作用，而大麻色烯（CBC）和大麻二酚（CBD）在 20 和 200mg/kg 时分别表现出显著作用（p<0.01）。在 TST 中进一步证实了 Δ9-THC 和 CBC 的抗抑郁样作用。Δ9-THC 表现出相同的 U 形剂量反应，在 2.5mg/kg 时具有显著的抗抑郁样作用（p<0.05），而 CBC 导致在 40 和 80mg/kg 剂量时运动性显著剂量依赖性降低（p<0.01）。本研究结果表明，Δ9-THC 和其他大麻素具有抗抑郁样作用，因此可能有助于大麻的整体情绪提升特性。
