Sidhu A, Fishman P H
Biochem Biophys Res Commun. 1986 Jun 30;137(3):943-9. doi: 10.1016/0006-291x(86)90316-5.
The D-1 dopamine receptor was extracted from rat striatal membranes with 0.7% sodium cholate and 1 M NaCl. Pretreatment of the membranes with a D-1 specific agonist, inclusion of crude phospholipids in the solubilization buffer, and subsequent removal of the detergent led to a maximal extraction of 48% of the receptor binding sites. The D-1 antagonist, [125I]SCH 23982, bound to single class of sites with a Kd of 1.8 nM and a Bmax of 1.65 pmol/mg protein. The solubilized receptors retained the ability to discriminate between active and inactive enantiomers of agonists and antagonists selective for the D-1 receptor.
用0.7%胆酸钠和1M氯化钠从大鼠纹状体膜中提取D-1多巴胺受体。用D-1特异性激动剂预处理膜，在溶解缓冲液中加入粗制磷脂，随后去除去污剂，可使48%的受体结合位点得到最大程度的提取。D-1拮抗剂[125I]SCH 23982与一类位点结合，解离常数（Kd）为1.8 nM，最大结合容量（Bmax）为1.65 pmol/mg蛋白质。溶解的受体保留了区分对D-1受体有选择性的激动剂和拮抗剂的活性和非活性对映体的能力。
