School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Nov 15;301:122973. doi: 10.1016/j.saa.2023.122973. Epub 2023 Jun 3.
Lipid droplets (LDs) are unique organelles that control the lipid metabolism in cells. It has been identified that the generations of LDs derive from endoplasmic reticulum (ER) and they have closely related with amount of cellular activities for maintaining homeostasis. To further explore the detail interactions between LDs and ER, we have developed a novel polarity-sensitive fluorescent probe LP with distinct D-π-A-π-D framework and applied it to imaging LDs and ER with dual colors at the same time. Probe LP showed well red-shifted emissions with the increase fraction of water in the 1,4- dioxane due to ICT process. In biological imaging, probe LP could visualize LDs and ER with green and red fluorescence separately. Besides, the dynamic behaviors of LDs and ER were achieved using LP during the oleic acids and starvation stimulations. Therefore, probe LP is a valuable molecular tool for investigating the relationships of LDs and ER in various cellular activities.
脂滴（LDs）是一种独特的细胞器，控制着细胞内的脂质代谢。已经确定，LDs 的产生源自内质网（ER），它们与维持细胞内稳态的细胞活动数量密切相关。为了进一步探索 LDs 和 ER 之间的详细相互作用，我们开发了一种新型的具有独特 D-π-A-π-D 骨架的极性敏感荧光探针 LP，并将其应用于同时以双色对 LDs 和 ER 进行成像。由于 ICT 过程，探针 LP 在 1,4-二恶烷中水分比例增加时表现出良好的红移发射。在生物成像中，探针 LP 可以分别用绿色和红色荧光来可视化 LDs 和 ER。此外，在油酸和饥饿刺激过程中，使用 LP 可以实现 LDs 和 ER 的动态行为。因此，探针 LP 是研究各种细胞活动中 LDs 和 ER 之间关系的有价值的分子工具。
