A longitudinal observational study was carried out from January 2020 to July 2021 to assess the impact of subclinical mastitis (SCM) on reproductive performance and its association with uterine health of crossbred dairy cows. The California Mastitis Test (CMT) and cytobrush technique were used to screen subclinical mastitis and subclinical endometritis, respectively. Milk samples positive for subclinical mastitis were subjected to bacteriological analysis. Data from 84 clinically healthy cows collected and analyzed. The present study revealed a prevalence of subclinical mastitis of 51.2% (43 of 84). The mean days from calving to first service interval were significantly longer in subclinical mastitis positive cows than negative (control) cows (120.51 ± 24.5 and 85.15 ± 28.3, respectively) (P < 0.05). The mean number of services per conception was significantly higher in positive cows (2.51 ± 0.83) than in negative cows (1.59 ± 0.81) (P < 0.05). Lower conception and pregnancy rates at first services were observed in subclinical mastitis cows. Risk factors analysis revealed that prevalence of subclinical mastitis significantly differed with the parity and body condition score (P < 0.05). The current study revealed that subclinical mastitis was significantly and directly associated with subclinical endometritis (P < 0.05). Subclinical mastitis significantly decreased (P = 0.000) progesterone concentrations and increased (P = 0.001) the cortisol concentrations. were the most predominant bacterial isolates from subclinical mastitic milk, followed by coagulase negative staphylococci (CNS) and streptococci. This study concludes a high prevalence of subclinical mastitis caused by could inflict harmful effects on reproductive performance of dairy cows, emphasizing the relevance of mastitis control programs in dairy farms.