Yu Wanjie, Nan Xuemei, Schroyen Martine, Wang Yue, Zhou Mengting, Tang Xiangfang, Xiong Benhai
Precision Livestock and Nutrition Laboratory, Teaching and Research Centre (TERRA), Gembloux Agro-Bio Tech, University of Liège, Gembloux 5030, Belgium.
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skae366.
Milk contains microRNAs (miRNA) that are shielded by small extracellular vesicles (sEVs). Beyond variations among individuals, many factors including nutrition play a role in shaping miRNA expression profiles. This study is to explore milk-derived sEVs-miRNA variations induced by inulin supplementation in subclinical mastitis-suffering cows. Fourteen lactating cows diagnosed with subclinical mastitis were equally assigned to either an inulin or a control group. Apart from total mixed rations, cows in the inulin group were provided with 300 g/d inulin during the morning feeding, while the control group did not receive any supplement. Following 1 wk of adaptation and 5 wk of treatment, sEVs-miRNA were isolated from the milk of each cow. RNA is subjected to high-throughput sequencing and differentially expressed (DE) miRNA (P < 0.05 and ∣ log2FC∣> 1) were detected through bioinformatics analysis. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to examine the target genes of DE miRNA. A sum of 350 miRNA was discovered, including 332 in the control group and 249 in the inulin group. Among these, 9 miRNA showed differential expression within the 2 groups, including 3 upregulated and 6 downregulated in the inulin group. The DE miRNA participates in regulating organismal systems, cellular processes, and signal transduction, which may affect inflammatory response and milk production. Overall, our study provides insight into the micromolecular-level mechanism of inulin in alleviating subclinical mastitis in dairy cows.
牛奶中含有被小细胞外囊泡（sEVs）包裹的微小RNA（miRNA）。除个体差异外，包括营养在内的许多因素都在塑造miRNA表达谱方面发挥作用。本研究旨在探索在患亚临床乳腺炎的奶牛中，菊粉补充剂诱导的源自牛奶的sEVs-miRNA变化。将14头被诊断为亚临床乳腺炎的泌乳奶牛平均分为菊粉组和对照组。除全混合日粮外，菊粉组奶牛在早晨喂食时每天额外提供300克菊粉，而对照组不接受任何补充剂。经过1周的适应期和5周的治疗后，从每头奶牛的牛奶中分离出sEVs-miRNA。对RNA进行高通量测序，并通过生物信息学分析检测差异表达（DE）的miRNA（P < 0.05且∣log2FC∣> 1）。进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析，以检查DE miRNA的靶基因。共发现350种miRNA，其中对照组有332种，菊粉组有249种。其中，9种miRNA在两组间存在差异表达，菊粉组中有3种上调，6种下调。这些DE miRNA参与调节机体系统、细胞过程和信号转导，这可能会影响炎症反应和产奶量。总体而言，我们的研究为菊粉缓解奶牛亚临床乳腺炎的微观分子机制提供了见解。
