Biogen, 225 Binney St, Cambridge, MA 02142, USA.
Agios Pharmarceuticals, 88 Sidney St, Cambridge, MA 02139, USA.
Bioanalysis. 2023 Jul;15(14):845-859. doi: 10.4155/bio-2023-0059. Epub 2023 Jun 12.
Develop and validate a volumetric absorptive microsampling (VAMS)-based LC-MS/MS method to support the bioanalysis of amino acid and carboxylic acid biomarkers in mouse whole blood. Mouse whole blood was collected using a 10 μl VAMS device. The analytes in VAMS were extracted and analyzed using an LC-MS/MS method. The VAMS-based LC-MS/MS assay exhibited a linearity range of 10.0-10,000 ng/ml with acceptable precision and accuracy and consistent recovery. The analyte stability in mouse whole blood VAMS was shown for 7 days at ambient conditions and at -80°C, as well as with three freeze/thaw cycles. A simple and robust VAMS-based LC-MS/MS method was developed and further validated for simultaneous bioanalysis of nine biomarkers in mouse whole blood.
开发并验证一种基于容量吸收微采样（VAMS）的 LC-MS/MS 方法，以支持对小鼠全血中氨基酸和羧酸生物标志物的生物分析。使用 10μl VAMS 装置采集小鼠全血。采用 LC-MS/MS 方法对 VAMS 中的分析物进行提取和分析。基于 VAMS 的 LC-MS/MS 测定法表现出 10.0-10,000ng/ml 的线性范围，具有可接受的精密度和准确度以及一致的回收率。在环境条件和-80°C 下以及经过三个冻融循环后，在小鼠全血 VAMS 中的分析物稳定性可保持 7 天。开发了一种简单而强大的基于 VAMS 的 LC-MS/MS 方法，并进一步验证了用于同时分析小鼠全血中 9 种生物标志物的方法。
Zotero 插件