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基于溶剂辅助风味蒸发富集的茶香气分析。

Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment.

机构信息

Tea Research Institute Chinese Academy of Agricultural Sciences.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University.

出版信息

J Vis Exp. 2023 May 26(195). doi: 10.3791/65522.

Abstract

Tea aroma is an important factor in tea quality, but it is challenging to analyze due to the complexity, low concentration, diversity, and lability of the volatile components of tea extract. This study presents a method for obtaining and analyzing the volatile components of tea extract with odor preservation using solvent-assisted flavor evaporation (SAFE) and solvent extraction followed by gas chromatography-mass spectrometry (GC-MS). SAFE is a high-vacuum distillation technique that can isolate volatile compounds from complex food matrices without any non-volatile interference. A complete step-by-step procedure for tea aroma analysis is presented in this article, including the tea infusion preparation, solvent extraction, SAFE distillation, extract concentration, and analysis by GC-MS. This procedure was applied to two tea samples (green tea and black tea), and qualitative as well as quantitative results on the volatile composition of the tea samples were obtained. This method can not only be used for the aroma analysis of various types of tea samples but also for molecular sensory studies on them.

摘要

茶香气是茶叶品质的一个重要因素,但由于茶叶提取物中挥发性成分的复杂性、低浓度、多样性和不稳定性,分析起来具有挑战性。本研究提出了一种使用溶剂辅助风味蒸发(SAFE)和溶剂萃取后气相色谱-质谱联用(GC-MS)获得并分析具有香气保留的茶叶提取物中挥发性成分的方法。SAFE 是一种高真空蒸馏技术,可从复杂的食品基质中分离挥发性化合物,而不会有任何非挥发性干扰。本文介绍了一种完整的茶叶香气分析步骤,包括茶浸提液的制备、溶剂萃取、SAFE 蒸馏、提取物浓缩以及 GC-MS 分析。该方法应用于两种茶叶样品(绿茶和红茶),并获得了茶叶样品挥发性成分的定性和定量结果。该方法不仅可用于各种类型的茶叶样品的香气分析,也可用于对它们的分子感官研究。

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