College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China; College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Virology. 2023 Aug;585:72-77. doi: 10.1016/j.virol.2023.05.008. Epub 2023 Jun 3.
The 3' untranslated region (UTR) of Senecavirus A (SVA) was predicted to harbor two hairpin structures, hairpin-I and -II. The former is composed of two internal loops, one terminal loop and three stem regions; the latter comprises one internal loop, one terminal loop and two stem regions. In this study, we constructed a total of nine SVA cDNA clones, which contained different point mutations within a stem-formed motif in the hairpin-I or -II, for rescuing replication-competent viruses. Only three mutants were successfully rescued and moreover genetically stable during at least five serial passages. Computer-aided prediction showed these three mutants bearing either a wild-type or a wild-type-like hairpin-I in their individual 3' UTRs. Neither wild-type nor wild-type-like hairpin-I could be computationally predicted to exist in 3' UTRs of the other six unviable "viruses". The results suggested that the wild-type or wild-type-like hairpin-I was necessary in the 3' UTR for SVA replication.
塞尼卡病毒 A（SVA）的 3' 非翻译区（UTR）被预测含有两个发夹结构，发夹-I 和 -II。前者由两个内部环、一个末端环和三个茎区组成；后者包含一个内部环、一个末端环和两个茎区。在这项研究中，我们构建了总共 9 个 SVA cDNA 克隆，它们在发夹-I 或 -II 中的茎形成基序内含有不同的点突变，用于拯救复制型病毒。只有三个突变体成功拯救出来，并且在至少五个连续传代中具有遗传稳定性。计算机辅助预测表明，这三个突变体在其各自的 3'UTR 中具有野生型或类似于野生型的发夹-I。在其他六个不能存活的“病毒”的 3'UTR 中，既不能预测到野生型也不能预测到野生型样的发夹-I 的存在。结果表明，野生型或野生型样的发夹-I 在 SVA 复制中是必需的。
