Bernstein J J, Goldberg W J
Brain Res. 1986 Jul 9;377(2):403-8. doi: 10.1016/0006-8993(86)90890-5.
Cell suspensions from 14-day gestation rat spinal cord can be successfully cultured on collagen gels containing laminin. After 7 and 14 days in culture the gels were removed from the dish and slices of gel with cultured cells were transplanted into the dorsal column of adult rats. Thirty days later there was no evidence of the gel. However, grafted neurons with well-developed organelles, axons, dendrites, axosomatic and axodendritic synapses were observed in the white matter of the host dorsal columns. Oligodendrocytes and astrocytes were also observed in the graft. This histocompatible substrate for culturing fetal CNS is a mechanism for transplanting cultured CNS cells as grafts of known maturity and synaptic organization into adult host CNS.
来自妊娠14天大鼠脊髓的细胞悬液能够成功地培养在含有层粘连蛋白的胶原凝胶上。培养7天和14天后，将凝胶从培养皿中取出，带有培养细胞的凝胶切片被移植到成年大鼠的背柱中。30天后，没有凝胶存在的迹象。然而，在宿主背柱的白质中观察到了细胞器、轴突、树突、轴体和轴树突触发育良好的移植神经元。在移植物中也观察到了少突胶质细胞和星形胶质细胞。这种用于培养胎儿中枢神经系统的组织相容性基质是一种将培养的中枢神经系统细胞作为已知成熟度和突触组织的移植物移植到成年宿主中枢神经系统中的机制。
