Brysk M M, Miller J, Chen S J, Moller P C, Stach R W
Cell Tissue Res. 1986;245(1):215-21. doi: 10.1007/BF00218103.
Exposure of fibroblasts to tunicamycin has been found to be cytotoxic for transformed cells, but not for nontransformed cells. With two mouse epidermal cell lines of common origin, we observe a contrary pattern: The malignant cells are more resistant to tunicamycin than their nonmalignant counterparts, as measured by growth and viability. With respect to the glycosylation of sugar precursors, the incorporation of mannose is more inhibited than that of glucosamine, while fucose is least impacted. Sugar incorporation is less reduced for the malignant cells, by a factor of two for fucose and more modestly for the other two sugars. There are no significant morphological changes; in particular, the desmosomal junctions are not affected. On polyacrylamide gels, we note intensity variations in several protein bands in response to tunicamycin, but little difference between malignant and nonmalignant cells when using either Coomassie stains or Concanavalin A overlays.
已发现将成纤维细胞暴露于衣霉素对转化细胞具有细胞毒性，但对未转化细胞则无毒性。对于源自共同来源的两种小鼠表皮细胞系，我们观察到一种相反的模式：通过生长和活力测量，恶性细胞比其非恶性对应细胞对衣霉素更具抗性。关于糖前体的糖基化，甘露糖的掺入比葡糖胺的掺入受到更大抑制，而岩藻糖受到的影响最小。恶性细胞的糖掺入减少程度较小，岩藻糖减少两倍，其他两种糖减少程度较小。没有明显的形态学变化；特别是，桥粒连接不受影响。在聚丙烯酰胺凝胶上，我们注意到响应衣霉素时几条蛋白带的强度变化，但使用考马斯亮蓝染色或伴刀豆球蛋白A覆盖时，恶性细胞和非恶性细胞之间几乎没有差异。
