Wang Fei, Wang Ying, Guo Xichun
Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, Weifang, People's Republic of China.
Department of Medical Record Room, Affiliated Hospital of Weifang Medical University, Weifang, People's Republic of China.
J Inflamm Res. 2023 Jun 8;16:2437-2446. doi: 10.2147/JIR.S409679. eCollection 2023.
Carotid artery restenosis (CAS) is a leading contributor to cerebrovascular diseases and one of the leading causes of death in the world. The purpose of this study was to assess the predictive efficiency of long non-coding RNA (lncRNA) TNFalpha-and hnRNP L-related immunoregulatory lncRNA (THRIL) and its association with the pathogenesis of CAS.
The expression of THRIL was determined in patients with asymptomatic CAS and human aortic endothelial cell (HAEC) models induced by oxidized low-density lipoprotein (ox-LDL). The receiver operating characteristic (ROC) curve and Kaplan-Meier (K-M) drawings were constructed to predict the risk of poor prognosis in patients with CAS. The cell proliferation, death rate, and inflammation were detected by 3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), flow cytometry, and enzyme-linked immunosorbent assay (ELISA) assays.
The relative expression of THRIL was elevated in patients with asymptomatic CAS. The findings of ROC curve indicated that THRIL had a predictive possibility on CAS. K-M finding and Cox regression analysis showed that the expression of THRIL and the degree of CAS were independent risk factors for poor prognosis in patients with CAS. THRIL was up-expressed in HAECs induced by ox-LDL. Down-regulation of THRIL could promote the proliferation of HAECs, inhibit cell apoptosis, and restrict cell inflammation.
THRIL was a diagnostic and prognostic biomarker in CAS and played an important role in regulating the proliferation, apoptosis, and inflammation of HAECs induced by ox-LDL.
颈动脉粥样硬化（CAS）是脑血管疾病的主要诱因之一，也是全球主要死因之一。本研究旨在评估长链非编码RNA（lncRNA）肿瘤坏死因子α和异质性核糖核蛋白L相关免疫调节lncRNA（THRIL）的预测效能及其与CAS发病机制的关联。
测定无症状CAS患者及氧化型低密度脂蛋白（ox-LDL）诱导的人主动脉内皮细胞（HAEC）模型中THRIL的表达。绘制受试者工作特征（ROC）曲线和Kaplan-Meier（K-M）图，以预测CAS患者预后不良的风险。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴盐（MTT）、流式细胞术和酶联免疫吸附测定（ELISA）检测细胞增殖、死亡率和炎症反应。
无症状CAS患者中THRIL的相对表达升高。ROC曲线结果表明THRIL对CAS具有预测可能性。K-M分析和Cox回归分析表明，THRIL的表达和CAS程度是CAS患者预后不良的独立危险因素。在ox-LDL诱导的HAECs中THRIL表达上调。THRIL的下调可促进HAECs增殖，抑制细胞凋亡，并限制细胞炎症。
THRIL是CAS的诊断和预后生物标志物，在调节ox-LDL诱导的HAECs增殖、凋亡和炎症中起重要作用。
