Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification.

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Colored wheat has piqued the interest of breeders and consumers alike. The chromosomal segment from 7E of , which carries a leaf rust resistant gene, , has been rarely employed in wheat breeding operations due to its association with the gene, which gives a yellow tint to the flour. By prioritizing nutritional content over color preferences, consumer acceptance has undergone a paradigm change. Through marker-assisted backcross breeding, we introduced an alien segment harboring the () gene into a high yielding commercial bread wheat (HD 2967) background to generate rust resistant carotenoid biofortified bread wheat. Agro-morphological characterization was also performed on a subset of developed 70 lines having enhanced grain carotene content. In the introgression lines, carotenoid profiling using HPLC analysis demonstrated a considerable increase in β-carotene levels (up to 12 ppm). Thus, the developed germplasm caters the threat to nutritional security and can be utilized to produce carotenoid fortified wheat.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s11032-022-01338-0.