Department of Nephrology, the First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.
Synapse Research Institute Maastricht, Maastricht, The Netherlands.
Platelets. 2023 Dec;34(1):2224454. doi: 10.1080/09537104.2023.2224454.
The anucleate human platelets contain a broad pattern of mRNAs and other RNA transcripts. The high quantitative similarity of mRNAs in megakaryocytes and platelets from different sources points to a common origin, and suggests a random redistribution of mRNA species upon proplatelet formation. A comparison of the classified platelet transcriptome (17.6k transcripts) with the identified platelet proteome (5.2k proteins) indicates an under-representation of: nuclear but not of other organellar proteins; membrane receptors and channels with low transcript levels; transcription/translation proteins; and so far uncharacterized proteins. In this review, we discuss the technical, normalization and database-dependent possibilities and challenges to come to a complete, genome-wide platelet transcriptome and proteome. Such a reference transcriptome and proteome can serve to further elucidate intra-subject and inter-subject differences in platelets in health and disease. Applications may also lay in the aid of genetic diagnostics.
无核的人类血小板含有广泛的 mRNA 和其他 RNA 转录本。不同来源的巨核细胞和血小板中的 mRNA 具有高度的定量相似性，这指向了一个共同的起源，并提示在血小板形成过程中 mRNA 种类的随机再分配。将分类的血小板转录组（17600 个转录本）与已鉴定的血小板蛋白质组（5200 个蛋白质）进行比较，表明存在以下代表性不足：核蛋白而非其他细胞器蛋白；低转录水平的膜受体和通道；转录/翻译蛋白；以及迄今尚未表征的蛋白质。在这篇综述中，我们讨论了技术、归一化和数据库依赖性的可能性和挑战，以获得完整的、全基因组的血小板转录组和蛋白质组。这样的参考转录组和蛋白质组可以进一步阐明健康和疾病状态下血小板在个体内和个体间的差异。其应用也可能在于遗传诊断的辅助。
