The role of the Peyer's patch in carcinogenesis. I. The adsorption from the gut and retention of 3-methylcholanthrene by Peyer's patches.

Radiotracer methods were used to determine the distribution of 3-methylcholanthrene (3-MC) within the lymphoid organs of rats following i.g. intubation, i.l. injection into the small intestine, i.v. injection or direct injection of the Peyer's patches with 3-[6-14C]methylcholanthrene (14C-MC). The data indicate that the gut-associated Peyer's patches and mesenteric lymph nodes were exposed to higher amounts of orally administered 14C-MC than any of the other lymphoid organs. Whereas the Peyer's patches exhibited the highest sp. act. for longer periods of time when low amounts of 14C-MC were administered, the sp. act. of the mesenteric lymph node were greater when rats were intubated with higher amounts of 14C-MC. Furthermore, the Peyer's patches were exposed to higher amounts of possible metabolites of 14C-MC. Injection of 14C-MC into the small intestinal lumen resulted in increased ratios of the Peyer's patch sp. act. to mesenteric lymph node sp. act., indicating that by-passing the stomach altered the distribution patterns. Data from rats injected i.v. with 14C-MC demonstrated that mesenteric lymph nodes but not Peyer's patches adsorbed and retained 14C-MC from the blood and indicated that the 14C-MC associated with Peyer's patches of i.g. treated rats was adsorbed from the gut rather than from the blood. Results obtained from rats which were exposed to 3-MC by directly injecting Peyer's patches with 14C-MC also indicated that the Peyer's patches were able to retain 3-MC once localized within this lymphoid organ, to metabolize the 3-MC and to possibly excrete the polycyclic aromatic hydrocarbon into the small intestine. Collectively the data indicate that Peyer's patches have an important role in the adsorption from the gut and subsequent retention of 3-MC and hence may be a likely target organ for lymphoid carcinogenesis following oral exposure to carcinogenic polycyclic aromatic hydrocarbons.