Zannella Carla, Chianese Annalisa, Annunziata Giuseppe, Ambrosino Annalisa, De Filippis Anna, Tenore Gian Carlo, Novellino Ettore, Stornaiuolo Mariano, Galdiero Massimiliano
Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy.
Microorganisms. 2023 May 20;11(5):1346. doi: 10.3390/microorganisms11051346.
Herpes simplex virus (HSV) is widespread in the population, causing oral or genital ulcers and, rarely, severe complications such as encephalitis, keratitis, and neonatal herpes. Current available anti-HSV drugs are acyclovir and its derivatives, although long-term therapy with these agents can lead to drug resistance. Thus, the discovery of novel antiherpetic compounds merits additional studies. In recent decades, much scientific effort has been invested in the discovery of new synthetic or natural compounds with promising antiviral properties. In our study, we tested the antiviral potential of a novel polyphenol-based nutraceutical formulation (named Taurisolo) consisting of a water polyphenol extract of grape pomace. The evaluation of the antiviral activity was carried out by using HSV-1 and HSV-2 in plaque assay experiments to understand the mechanism of action of the extract. Results were confirmed by real-time PCR, transmission electron microscope (TEM), and fluorescence microscope. Taurisolo was able to block the viral infection by acting on cells when added together with the virus and also when the virus was pretreated with the extract, demonstrating an inhibitory activity directed to the early phases of HSV-1 and HSV-2 infection. Altogether, these data evidence for the first time the potential use of Taurisolo as a topical formulation for both preventing and healing herpes lesions.
单纯疱疹病毒（HSV）在人群中广泛传播，可引起口腔或生殖器溃疡，极少数情况下会导致严重并发症，如脑炎、角膜炎和新生儿疱疹。目前可用的抗HSV药物是阿昔洛韦及其衍生物，尽管长期使用这些药物会导致耐药性。因此，发现新型抗疱疹化合物值得进一步研究。近几十年来，人们投入了大量科学精力来发现具有潜在抗病毒特性的新合成或天然化合物。在我们的研究中，我们测试了一种新型基于多酚的营养制剂（名为Taurisolo）的抗病毒潜力，该制剂由葡萄皮渣的水多酚提取物组成。通过在噬斑试验中使用HSV-1和HSV-2来评估抗病毒活性，以了解提取物的作用机制。结果通过实时PCR、透射电子显微镜（TEM）和荧光显微镜得到证实。当与病毒一起添加时，以及当病毒用提取物预处理时，Taurisolo能够通过作用于细胞来阻断病毒感染，这表明其对HSV-1和HSV-2感染的早期阶段具有抑制活性。总之，这些数据首次证明了Taurisolo作为预防和治愈疱疹病变的局部制剂的潜在用途。
