Glassman A B, Bennett C E
Transfusion. 1979 Mar-Apr;19(2):178-81. doi: 10.1046/j.1537-2995.1979.19279160289.x.
Successful cryopreservation of human lymphocytes has been previously described. Cryopreserved lymphocytes are useful for a variety of in vitro immunologic studies. This study was performed to determine the applicability and/or advantages of using a programmable freezing system, and compares glycerol versus dimethyl sulfoxide (DMSO) at varying concentrations on post-thaw viability, E-rosetting and immunoglobulin fluorescence. Prefreeze T and B lymphocyte percentages were determined. Cells were then frozen in varying concentrations of glycerol and DMSO. Optimum cryoprotectant type and concentration was determined. Lymphocytes from seven individuals were frozen by the batch method in a mechanical freezer and with the automated liquid nitrogen injection system. Data on post-thaw T and B percentages and viability revealed 10% DMSO and liquid nitrogen control freezing method at 1 C/minute as the best conditions for lymphocyte preservation as reflected by post-thaw in vitro testing.
此前已有关于人类淋巴细胞成功冻存的描述。冻存的淋巴细胞可用于多种体外免疫学研究。本研究旨在确定使用可编程冷冻系统的适用性和/或优势，并比较不同浓度的甘油与二甲基亚砜（DMSO）对解冻后活力、E 花环形成及免疫球蛋白荧光的影响。测定了冻存前 T 淋巴细胞和 B 淋巴细胞的百分比。然后将细胞置于不同浓度的甘油和 DMSO 中进行冻存。确定了最佳冷冻保护剂类型和浓度。来自七名个体的淋巴细胞通过分批法在机械冷冻机以及自动液氮注射系统中进行冻存。解冻后 T 淋巴细胞和 B 淋巴细胞百分比及活力的数据显示，解冻后体外测试表明，10% DMSO 以及液氮控制冷冻法（1℃/分钟）是淋巴细胞保存的最佳条件。
