Glassman A B, Christopher J B
Transfusion. 1984 Nov-Dec;24(6):538-9. doi: 10.1046/j.1537-2995.1984.24685066822.x.
Lymphocytes were frozen at a density of 5 to 10 X 10(6) cells per ml at 1 degree C per minute in a programmable liquid nitrogen freezer. T-subsets were determined by using monoclonal antibodies. A fluorescein-conjugated polyvalent antisera with B-cell specificity was used to define B-cells. No significant change in numbers of total T-lymphocytes or -subsets was identified in the frozen lymphocytes when compared to fresh controls. Liquid nitrogen cryopreservation is an acceptable method of storing lymphocytes for subsequent determination of T-subsets using monoclonal antibodies.
淋巴细胞在可编程液氮冷冻机中以每分钟1℃的速度冷冻，密度为每毫升5至10×10⁶个细胞。通过使用单克隆抗体来测定T细胞亚群。使用具有B细胞特异性的荧光素偶联多价抗血清来定义B细胞。与新鲜对照相比，冷冻淋巴细胞中总T淋巴细胞或其亚群的数量没有显著变化。液氮冷冻保存是一种可接受的储存淋巴细胞的方法，用于随后使用单克隆抗体测定T细胞亚群。
