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[Nonhomogeneity of the distribution of the absorbing substance in cytophotometry].

作者信息

Gruzdev A D, Sherudilo A I

出版信息

Tsitologiia. 1979 Feb;21(2):227-30.

PMID:373201
Abstract

Results of two-wavelengths cytophotometry may be used to calculate m--the quantity of light absorbing substance, and gamma--the degree of non-homogeneity of distribution of the substance on a preparation. Formula (1) is almost free from any distributional error. Optical density limits of applicability of formula (3) for gamma are shown on fig. 2. An algorithm for calculation of gamma at higher optical densities is proposed.

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