Gruzdev A D, Sherudilo A I
Tsitologiia. 1979 Feb;21(2):227-30.
Results of two-wavelengths cytophotometry may be used to calculate m--the quantity of light absorbing substance, and gamma--the degree of non-homogeneity of distribution of the substance on a preparation. Formula (1) is almost free from any distributional error. Optical density limits of applicability of formula (3) for gamma are shown on fig. 2. An algorithm for calculation of gamma at higher optical densities is proposed.
双波长细胞光度测定法的结果可用于计算m(吸光物质的量)和γ(该物质在制剂上分布的不均匀程度)。公式(1)几乎不存在任何分布误差。图2显示了公式(3)对γ的适用光学密度极限。本文提出了一种在较高光学密度下计算γ的算法。