Choudhury Sumana, Anne Anuhya, Pradhan Purnima P, Mohan K Naga
Molecular Biology and Genetics Laboratory, Department of Biological Sciences, BITS Pilani Hyderabad Campus, Hyderabad, India; Centre for Human Disease Research, BITS Pilani Hyderabad Campus, Hyderabad, India.
Molecular Biology and Genetics Laboratory, Department of Biological Sciences, BITS Pilani Hyderabad Campus, Hyderabad, India.
Stem Cell Res. 2023 Sep;71:103141. doi: 10.1016/j.scr.2023.103141. Epub 2023 Jun 10.
DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC line showed increased transcript levels of Sox2 pluripotency marker. R1 embryoid bodies showed increased levels of Lefty1 (endoderm), Tbxt and Acta2 (mesoderm), and Pax6 (ectoderm) transcripts. This new line showed normal karyotype and microsatellite profiles making it useful in studying carcinogenesis and abnormal neurogenesis due to DNMT1 overexpression.
据报道，在精神分裂症、双相情感障碍、癫痫和多种癌症类型等疾病中存在DNMT1过表达。在此，我们利用非同源重组产生了R1，这是一种携带Dnmt1 cDNA转基因的小鼠胚胎干细胞（ESC）系，以实现约两倍的过表达。该ESC系显示多能性标记物Sox2的转录水平增加。R1类胚体显示Lefty1（内胚层）、Tbxt和Acta2（中胚层）以及Pax6（外胚层）转录物水平增加。这个新细胞系显示出正常的核型和微卫星图谱，使其在研究由于DNMT1过表达导致的致癌作用和异常神经发生方面很有用。
