Lopez J, Ogren L, Talamantes F
Endocrinology. 1986 Sep;119(3):1020-7. doi: 10.1210/endo-119-3-1020.
The effects of neonatal diethylstilbestrol (DES) treatment on pituitary PRL response to dopamine (DA) inhibition or estrogen stimulation were studied in C3H/MTV+ mice at 10 weeks, 5 months and 8 months of age using synthesis and release of [3H]PRL by the pituitary as endpoints. In the DA study, anterior pituitaries from ovariectomized or intact, neonatally hormone-treated mice were incubated for 6 h in the presence or absence of DA. In the estrogen study, serum and anterior pituitaries were obtained from ovariectomized neonatally hormone-treated mice injected with oil or 17 beta-estradiol for 7 days before death. Neonatal treatment of mice with 2.5 micrograms/day DES altered basal [3H]PRL synthesis and release as well as circulating PRL concentrations. The results of analysis of variance indicated a significant interaction between the effects of neonatal hormone treatment and DA treatment on [3H]PRL release. Neonatal DES treatment did not alter the response of pituitary PRL to estrogen treatment at the ages examined.
在10周龄、5月龄和8月龄的C3H/MTV +小鼠中，以垂体[3H]PRL的合成与释放为终点，研究了新生期己烯雌酚（DES）处理对垂体PRL对多巴胺（DA）抑制或雌激素刺激反应的影响。在DA研究中，将来自新生期经激素处理的去卵巢或完整小鼠的垂体前叶在有或无DA的情况下孵育6小时。在雌激素研究中，从新生期经激素处理的去卵巢小鼠中获取血清和垂体前叶，这些小鼠在处死前7天注射油或17β-雌二醇。用2.5微克/天的DES对新生小鼠进行处理，改变了基础[3H]PRL的合成与释放以及循环PRL浓度。方差分析结果表明，新生期激素处理和DA处理对[3H]PRL释放的影响之间存在显著交互作用。在所研究的年龄阶段，新生期DES处理并未改变垂体PRL对雌激素处理的反应。
