Chen Lei, Wu Fen, Xiang Ming, Zhang Wenna, Wu Qingxi, Lu Yongming, Fu Jiajun, Chen Meilu, Li Songnan, Chen Yan, Du Xianfeng
Anhui Key Laboratory of Ecological Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.
Int J Biol Macromol. 2023 Aug 1;245:125245. doi: 10.1016/j.ijbiomac.2023.125245. Epub 2023 Jun 15.
Starch-based composite nanofibrous films loaded with tea polyphenols (TP) were successfully fabricated through electrospinning high amylose corn starch (HACS) with aid of polyvinyl alcohol (PVA), referred as HACS/PVA@TP. With the addition of 15 % TP, HACS/PVA@TP nanofibrous films exhibited enhanced mechanical properties and water vapor barrier capability, and their hydrogen bonding interactions were further evidenced. TP was slowly released from the nanofibrous film and followed Fickian diffusion mechanism, which achieved the controlled sustained release of TP. Interesting, HACS/PVA@TP nanofibrous films effectively improved antimicrobial activities against Staphylococcus aureus (S. aureus) and prolonged the shelf life of strawberry. HACS/PVA@TP nanofibrous films showed superior antibacterial function by by destroying cell wall and cytomembrane, and degrading existing DNA fragments, stimulating excessive intracellular reactive oxygen species (ROS) generation. Our study demonstrated that the functional electrospun Starch-based nanofibrous films with enhanced mechanical properties and superior antimicrobial activities were potential for the application in active food packaging and relative areas.
通过在高直链玉米淀粉（HACS）中加入聚乙烯醇（PVA），借助静电纺丝成功制备了负载茶多酚（TP）的淀粉基复合纳米纤维膜，即HACS/PVA@TP。添加15%的TP后，HACS/PVA@TP纳米纤维膜的机械性能和水蒸气阻隔能力增强，且其氢键相互作用得到进一步证明。TP从纳米纤维膜中缓慢释放，遵循菲克扩散机制，实现了TP的可控缓释。有趣的是，HACS/PVA@TP纳米纤维膜有效提高了对金黄色葡萄球菌（S. aureus）的抗菌活性，并延长了草莓的保质期。HACS/PVA@TP纳米纤维膜通过破坏细胞壁和细胞膜、降解现有DNA片段、刺激细胞内过量活性氧（ROS）生成，表现出优异的抗菌功能。我们的研究表明，具有增强机械性能和优异抗菌活性的功能性静电纺丝淀粉基纳米纤维膜在活性食品包装及相关领域具有应用潜力。
