Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea; Center for Wave Energy Materials, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
J Mol Biol. 2023 Jun 1;435(11):167975. doi: 10.1016/j.jmb.2023.167975. Epub 2023 Jun 16.
Single-molecule force spectroscopy is a unique method that can probe the structural changes of single proteins at a high spatiotemporal resolution while mechanically manipulating them over a wide force range. Here, we review the current understanding of membrane protein folding learned by using the force spectroscopy approach. Membrane protein folding in lipid bilayers is one of the most complex biological processes in which diverse lipid molecules and chaperone proteins are intricately involved. The approach of single protein forced unfolding in lipid bilayers has produced important findings and insights into membrane protein folding. This review provides an overview of the forced unfolding approach, including recent achievements and technical advances. Progress in the methods can reveal more interesting cases of membrane protein folding and clarify general mechanisms and principles.
单分子力谱学是一种独特的方法，可以在高时空分辨率下探测单个蛋白质的结构变化，同时在广泛的力范围内对其进行机械操作。在这里，我们回顾了使用力谱学方法了解到的膜蛋白折叠的最新认识。在脂质双层中，膜蛋白的折叠是最复杂的生物过程之一，其中涉及多种脂质分子和伴侣蛋白。在脂质双层中单蛋白强制展开的方法已经产生了关于膜蛋白折叠的重要发现和见解。本综述概述了强制展开方法，包括最新的成果和技术进展。该方法的进展可以揭示更多有趣的膜蛋白折叠案例，并阐明一般机制和原理。
