Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China.
Food Chem. 2023 Nov 15;426:136615. doi: 10.1016/j.foodchem.2023.136615. Epub 2023 Jun 12.
Pickering emulsion catalytic system (PEC) stabilized by nanoparticles is an efficient catalytic platform. Herein, a high-performance PEC was constructed by acetylated modification of arachin nanoparticles (AAPs). The results showed the pI of arachin was decreased from pH 5.5 to pH 3.5. The surface hydrophobicity index was significantly increased (from 56.28 ± 4.23 to 120.77 ± 0.79) after acetylated modification. The three-phase contact angle of AAPs was 91.20 ± 0.98°. AAPs were used as lipase immobilization carriers to increase the activity of free lipase fabricating lipase-AAPs. The immobilization efficiency and activity of lipase-AAPs were 12.95 ± 0.03% and 1.74 ± 0.07 U/mg, respectively. Enzymatic reaction kinetics showed that V of lipase-AAPs was twice of free lipase. K was 1/5 of free lipase. The catalytic efficiency of PEC to prepare DAG was 2.36 times of biphasic catalytic system (BCS). This work provided a promising way to promote the efficiency of DAG preparation.
纳米粒子稳定的 Pickering 乳液催化体系（PEC）是一种高效的催化平台。在此，通过对 arachin 纳米粒子（AAPs）进行乙酰化修饰，构建了一种高性能的 PEC。结果表明，arachin 的等电点从 pH 5.5 降低到 pH 3.5。乙酰化修饰后，表面疏水性指数显著增加（从 56.28 ± 4.23 增加到 120.77 ± 0.79）。AAPs 的三相接触角为 91.20 ± 0.98°。AAPs 可用作脂肪酶固定化载体，以提高游离脂肪酶制备脂肪酶-AAPs 的活性。脂肪酶-AAPs 的固定化效率和活性分别为 12.95 ± 0.03%和 1.74 ± 0.07 U/mg。酶促反应动力学表明，脂肪酶-AAPs 的 V 是游离脂肪酶的两倍。K 是游离脂肪酶的 1/5。PEC 制备 DAG 的催化效率是两相催化体系（BCS）的 2.36 倍。这项工作为提高 DAG 制备效率提供了一种有前途的方法。
