Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Pathology, Shandong Province, China.
Gao Tang People's Hospital Affiliated to Jining Medical University, Emergency Department, Shandong Province, China.
Braz J Otorhinolaryngol. 2023 Jul-Aug;89(4):101278. doi: 10.1016/j.bjorl.2023.101278. Epub 2023 Jun 3.
To investigate the effect of peroxynitrite on the cultured cochlear hair cells of C57BL/6 P3 mice in vitro as well as the role of Wnt3a, as an activator of the canonical Wnt signaling pathway, underlying the action of such an oxidative stress.
The in vitro primary cultured cochlear hair cells were subjected to l00 μM peroxynitrite and l00 μM peroxynitrite +25 ng/mL Wnt3a for 24 h, the cell survival and morphological changes were examined by immunofluorescence and transmission electron microscopy.
The number of surviving hair cells was significantly reduced in the 100 μM peroxynitrite group, while it was significantly higher in the Wnt3a + peroxynitrite treated group compared with the peroxynitrite treated group. The transmission electron microscopy showed that exposure to peroxynitrite induced a dramatic decrease in the number of mitochondria and severely disrupted mitochondrial ultrastructure, while Wnt3a clearly diminished the disruption of mitochondrial structure and preserved a higher number of mitochondria.
These results indicated that peroxynitrite could cause oxidative damage to the cochlear hair cells, and low concentrations of Wnt3a has a protective effect against oxidative damage.
Level 2.
研究过氧亚硝酸盐对体外培养的 C57BL/6 P3 小鼠耳蜗毛细胞的影响，以及作为经典 Wnt 信号通路激活剂的 Wnt3a 在这种氧化应激作用下的作用。
对体外原代培养的耳蜗毛细胞进行 100μM 过氧亚硝酸盐和 100μM 过氧亚硝酸盐+25ng/mL Wnt3a 处理 24h，通过免疫荧光和透射电子显微镜观察细胞存活和形态变化。
100μM 过氧亚硝酸盐组的存活毛细胞数量明显减少，而 Wnt3a+过氧亚硝酸盐处理组的存活毛细胞数量明显高于过氧亚硝酸盐处理组。透射电子显微镜显示，过氧亚硝酸盐暴露导致线粒体数量急剧减少，线粒体超微结构严重破坏，而 Wnt3a 明显减轻了线粒体结构的破坏，并保留了更多的线粒体。
这些结果表明，过氧亚硝酸盐可引起耳蜗毛细胞氧化损伤，低浓度的 Wnt3a 对氧化损伤具有保护作用。
2 级。
