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利用硫酸铵水溶液延长单核苷酸通过固态纳米孔的驻留时间并进行识别

Dwell Time Prolongation and Identification of Single Nucleotides Passing through a Solid-State Nanopore by Using Ammonium Sulfate Aqueous Solution.

作者信息

Yanagi Itaru, Akahori Rena, Takeda Ken-Ichi

机构信息

Center for Exploratory Research, Research & Development Group, Hitachi, Ltd., 1-280, Higashi-koigakubo, Kokubunji, Tokyo 185-8603, Japan.

Center for Technology Innovation - Healthcare, Research & Development Group, Hitachi, Ltd., 1-280, Higashi-koigakubo, Kokubunji, Tokyo 185-8603, Japan.

出版信息

ACS Omega. 2023 Jun 1;8(23):21285-21292. doi: 10.1021/acsomega.3c02703. eCollection 2023 Jun 13.

DOI:10.1021/acsomega.3c02703
PMID:37332803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10268630/
Abstract

The ionic current blockades when poly(dT) or dNTPs passed through SiN nanopores in an aqueous solution containing (NH)SO were investigated. The dwell time of poly(dT) in the nanopores in an aqueous solution containing (NH)SO was significantly longer compared to that in an aqueous solution that did not contain (NH)SO. This dwell time prolongation effect due to the aqueous solution containing (NH)SO was also confirmed when dCTP passed through the nanopores. In addition, when the nanopores were fabricated via dielectric breakdown in the aqueous solution containing (NH)SO, the dwell time prolongation effect for dCTP still occurred even after the aqueous solution was displaced with the aqueous solution without (NH)SO. Furthermore, we measured the ionic current blockades when the four types of dNTPs passed through the same nanopore, and the four types of dNTPs could be statistically identified according to their current blockade values.

摘要

研究了在含有(NH)₂SO₄的水溶液中，聚(dT)或脱氧核苷三磷酸(dNTPs)通过氮化硅(SiN)纳米孔时的离子电流阻断情况。与不含(NH)₂SO₄的水溶液相比，聚(dT)在含有(NH)₂SO₄的水溶液中的纳米孔中的停留时间显著更长。当dCTP通过纳米孔时，也证实了含有(NH)₂SO₄的水溶液所导致的这种停留时间延长效应。此外，当在含有(NH)₂SO₄的水溶液中通过介电击穿制造纳米孔时，即使在用不含(NH)₂SO₄的水溶液置换后，dCTP的停留时间延长效应仍然存在。此外，我们测量了四种类型的dNTPs通过同一个纳米孔时的离子电流阻断情况，并且可以根据它们的电流阻断值对这四种类型的dNTPs进行统计学识别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/2756090e704c/ao3c02703_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/7d5d67c5ef1d/ao3c02703_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/5e91ca80668c/ao3c02703_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/e9e2e7d81b20/ao3c02703_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/3ddaf6c4493f/ao3c02703_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/2756090e704c/ao3c02703_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/7d5d67c5ef1d/ao3c02703_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/5e91ca80668c/ao3c02703_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/e9e2e7d81b20/ao3c02703_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/3ddaf6c4493f/ao3c02703_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73da/10268630/2756090e704c/ao3c02703_0006.jpg

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Nanopore long-read-only metagenomics enables complete and high-quality genome reconstruction from mock and complex metagenomes.纳米孔长读长测序技术仅进行宏基因组学分析即可实现模拟和复杂宏基因组的完整、高质量的基因组重建。
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Sub-10-nm-thick SiN nanopore membranes fabricated using the SiOsacrificial layer process.使用SiO牺牲层工艺制造的厚度小于10纳米的氮化硅纳米孔膜。
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