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用于纳米孔测序、传感以及比较MspA突变体功能变异的生物纳米孔的最新进展。

Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.

作者信息

Bhatti Huma, Jawed Rohil, Ali Irshad, Iqbal Khurshid, Han Yan, Lu Zuhong, Liu Quanjun

机构信息

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University No. 2 Sipailou Nanjing 210096 People's Republic of China

School of Life Science and Technology, Southeast University No. 2 Sipailou Nanjing 210096 People's Republic of China.

出版信息

RSC Adv. 2021 Aug 31;11(46):28996-29014. doi: 10.1039/d1ra02364k. eCollection 2021 Aug 23.

DOI:10.1039/d1ra02364k
PMID:35478559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9038099/
Abstract

Biological nanopores are revolutionizing human health by the great myriad of detection and diagnostic skills. Their nano-confined area and ingenious shape are suitable to investigate a diverse range of molecules that were difficult to identify with the previous techniques. Additionally, high throughput and label-free detection of target analytes instigated the exploration of new bacterial channel proteins such as Fragaceatoxin C (FraC), Cytolysin A (ClyA), Ferric hydroxamate uptake component A (FhuA) and Curli specific gene G (CsgG) along with the former ones, like α-hemolysin (αHL), porin A (MspA), aerolysin, bacteriophage phi 29 and Outer membrane porin G (OmpG). Herein, we discuss some well-known biological nanopores but emphasize on MspA and compare the effects of site-directed mutagenesis on the detection ability of its mutants in view of the surface charge distribution, voltage threshold and pore-analyte interaction. We also discuss illustrious and latest advances in biological nanopores for past 2-3 years due to limited space. Last but not the least, we elucidate our perspective for selecting a biological nanopore and propose some future directions to design a customized nanopore that would be suitable for DNA sequencing and sensing of other nontrivial molecules in question.

摘要

生物纳米孔凭借其众多的检测和诊断技术正在彻底改变人类健康。它们的纳米受限区域和独特形状适合研究一系列以前的技术难以识别的分子。此外，对目标分析物的高通量和无标记检测促使人们探索新的细菌通道蛋白，如草莓毒素C（FraC）、溶细胞素A（ClyA）、高铁载体摄取成分A（FhuA）和卷曲菌毛特异性基因G（CsgG），以及以前的一些蛋白，如α-溶血素（αHL）、孔蛋白A（MspA）、气溶素、噬菌体phi 29和外膜孔蛋白G（OmpG）。在此，我们讨论一些著名的生物纳米孔，但重点介绍MspA，并从表面电荷分布、电压阈值和孔与分析物相互作用的角度比较定点诱变对其突变体检测能力的影响。由于篇幅有限，我们还讨论了过去两到三年生物纳米孔方面显著的和最新的进展。最后但同样重要的是，我们阐明了选择生物纳米孔的观点，并提出了一些未来的方向，以设计一种定制的纳米孔，该纳米孔适用于DNA测序和检测其他相关的重要分子。

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