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内酯酶介导的群体感应抑制在很大程度上改变了 E264 的表型、蛋白质组和抗菌活性。

Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in E264.

机构信息

Aix Marseille Université, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.

Gene&GreenTK, Marseille, France.

出版信息

Front Cell Infect Microbiol. 2023 Jun 2;13:1190859. doi: 10.3389/fcimb.2023.1190859. eCollection 2023.

DOI:10.3389/fcimb.2023.1190859
PMID:37333853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10272358/
Abstract

INTRODUCTION

is a study model for , a highly virulent pathogen, known to be the causative agent of melioidosis and a potential bioterrorism agent. These two bacteria use an (acyl-homoserine lactone) AHL-mediated quorum sensing (QS) system to regulate different behaviors including biofilm formation, secondary metabolite productions, and motility.

METHODS

Using an enzyme-based quorum quenching (QQ) strategy, with the lactonase Pox having the best activity on AHLs, we evaluated the importance of QS in by combining proteomic and phenotypic analyses.

RESULTS

We demonstrated that QS disruption largely affects overall bacterial behavior including motility, proteolytic activity, and antimicrobial molecule production. We further showed that QQ treatment drastically decreases bactericidal activity against two bacteria ( and ), while a spectacular increase in antifungal activity was observed against fungi and yeast (, and ).

DISCUSSION

This study provides evidence that QS is of prime interest when it comes to understanding the virulence of species and developing alternative treatments.

摘要

简介

是一种研究模型，是一种高度致命的病原体，已知是类鼻疽病的病原体，也是一种潜在的生物恐怖主义剂。这两种细菌使用（酰基高丝氨酸内酯）AHL 介导的群体感应（QS）系统来调节不同的行为，包括生物膜形成、次生代谢产物的产生和运动性。

方法

使用基于酶的群体感应淬灭（QQ）策略，具有最好的活性的内酯酶 Pox 对 AHLs，我们通过蛋白质组学和表型分析评估了 QS 在中的重要性。

结果

我们证明，QS 破坏在很大程度上影响了整体细菌行为，包括运动性、蛋白水解活性和抗菌分子的产生。我们进一步表明，QQ 处理大大降低了对两种细菌（和）的杀菌活性，而对真菌和酵母（、和）的抗真菌活性则显著增加。

讨论

本研究提供了证据，证明当涉及到了解物种的毒力和开发替代治疗方法时，QS 是首要关注的问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53c/10272358/171d6eb5d4ec/fcimb-13-1190859-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53c/10272358/ad61318f01bc/fcimb-13-1190859-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53c/10272358/171d6eb5d4ec/fcimb-13-1190859-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53c/10272358/00ff8af4a093/fcimb-13-1190859-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53c/10272358/356bbb55c820/fcimb-13-1190859-g006.jpg
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