Birch Julie Melsted, Leijon Mikael, Nielsen Søren Saxmose, Struve Tina, Jensen Henrik Elvang
Section for Pathobiological Sciences, Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark.
Kopenhagen Fur, 2600 Glostrup, Denmark.
FEMS Microbes. 2021 Apr 16;2:xtab005. doi: 10.1093/femsmc/xtab005. eCollection 2021.
Clarification of the infection microbiology remains a challenge in the pre-weaning diarrhea (PWD) syndrome in farmed mink (). Duodenal, jejunal and colon sections from 36 mink kits with PWD were systematically examined by chromogen hybridization targeting two incriminated viruses: mink astrovirus and mink sapovirus. Using the RNAscope 2.5 HD Duplex Assay, astrovirus and sapovirus were visualized and simultaneously demonstrated in the gut tissue. Both viruses infect enterocytes in the small intestine with a specific localization pattern; astrovirus affects the two apical thirds of the villi, whereas sapovirus generally affects the basal parts of the villi. Furthermore, we demonstrated that astrovirus in mink does not target the goblet cells. This is the first time astro- and calicivirus have been visualized in mink kit gut tissue, and these findings might be important in clarification of the impact of these viruses in the PWD syndrome.
在养殖水貂的断奶前腹泻（PWD）综合征中，感染微生物学的阐明仍然是一项挑战。对36只患有PWD的水貂幼崽的十二指肠、空肠和结肠切片进行了系统检查，采用针对两种致病病毒的显色杂交法：水貂星状病毒和水貂杯状病毒。使用RNAscope 2.5 HD双链分析，在肠道组织中可视化并同时显示了星状病毒和杯状病毒。两种病毒均以特定的定位模式感染小肠中的肠上皮细胞；星状病毒影响绒毛的顶部三分之二，而杯状病毒通常影响绒毛的基部。此外，我们证明水貂中的星状病毒不靶向杯状细胞。这是首次在水貂幼崽肠道组织中可视化星状病毒和杯状病毒，这些发现可能对阐明这些病毒在PWD综合征中的影响具有重要意义。
