Isoquinoline alkaloids isolated from var. and subsp. var. with bioactivity studies.

CONTEXT

The genus Mill., one of the important Papaveraceae family plants, is rich in isoquinoline alkaloids and distributed worldwide.

OBJECTIVE

Isolation and identification of bioactive alkaloids from Boiss. & Huet. subsp. (Nabelek) Mory var. (Cullen) Mory and (L.) Rudolph var. (Aslan 2012) and investigation of their antioxidant and anticholinesterase activities.

MATERIALS AND METHODS

The aerial parts of each plant were dried, powdered, and percolated with methanol, then each extract was fractionated between 50% aqueous acetic acid and petroleum. Their aqueous acidic layer was adjusted to pH 7-8 with NHOH and extracted with chloroform, the extract was subjected to CC separation and isolation. Structures of the isolated alkaloids were elucidated by 1D and 2D-NMR and mass spectral analyses. The alkaloid extracts and their pure alkaloids were tested for anti-cholinesterase (AChE and BuChE) and antioxidant (ABTS, CUPRAC, β-carotene linoleic acid tests) activities .

RESULTS

Methanol extracts of subsp. var. and var. afforded a novel compound glauciumoline and seven known isoquinoline alkaloids three of which have an aporphine-type and the other five have a protopine-type skeleton. Among them, -protopinium () and -protopinium () were isolated from a species for the first time. Tertiary amine extracts (TAEs) of both plants showed very strong acetylcholinesterase inhibitory activity. The TAE of the plants also showed strong antioxidant activity while the isolated alkaloids showed no meaningful activity in the anticholinesterase and antioxidant tests.

DISCUSSION AND CONCLUSIONS

species are considered promising therapeutic agents in the treatment of Alzheimer's disease.