基于毛细管电泳法的微流控芯片中荧光微球的直接计数。

Direct count of fluorescent microspheres in a microfluidic chip based on the capillary electrophoresis method.

机构信息

Anhui Sanlian University, Hefei 230000, China.

Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, University of Shanghai for Science and Technology, Shanghai 200093, China.

出版信息

Anal Methods. 2023 Jun 29;15(25):3014-3018. doi: 10.1039/d3ay00710c.

DOI:10.1039/d3ay00710c

PMID:37334474

Abstract

Fluorescent microspheres (FMs) are tiny particles with special functions that are widely employed in biological research. Counting of microscale FMs is a great challenge by capillary electrophoresis. Herein we developed a method to count 2 μm FMs based on a microfluidic chip with a gradual change in inner size. Such a microfluidic chip can inhibit sample blocking at the inlet of the capillary. The results showed that FMs migrated in the wide part of the microchannel side by side, and then passed through the narrow part one by one. There was a linear relationship between the number of peaks in the electropherogram and concentration of FMs if they were running in the microchannel for more than 20 min. A high separation voltage may lead to aggregation of FMs in the microchannels, and about 2 × 10 FMs can be counted within 30 min by this microfluidic chip.

摘要

荧光微球（FMs）是具有特殊功能的微小颗粒，广泛应用于生物研究。通过毛细管电泳对微尺度 FMs 进行计数是一项巨大的挑战。在此，我们开发了一种基于内尺寸逐渐变化的微流控芯片来对 2μm FMs 进行计数的方法。这种微流控芯片可以抑制毛细管入口处的样品堵塞。结果表明，FMs 在微通道的宽部分并排迁移，然后逐个通过窄部分。如果 FMs 在微通道中运行超过 20 分钟，则电泳图谱中的峰数与 FMs 的浓度之间存在线性关系。较高的分离电压可能导致 FMs 在微通道中聚集，通过这种微流控芯片可以在 30 分钟内计数约 2×10 FMs。