Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary.
Servier Research Institute of Medicinal Chemistry, Budapest, Hungary.
Biotechnol Bioeng. 2023 Oct;120(10):2793-2808. doi: 10.1002/bit.28469. Epub 2023 Jun 19.
Opines and opine-type chemicals are valuable natural products with diverse biochemical roles, and potential synthetic building blocks of bioactive compounds. Their synthesis involves reductive amination of ketoacids with amino acids. This transformation has high synthetic potential in producing enantiopure secondary amines. Nature has evolved opine dehydrogenases for this chemistry. To date, only one enzyme has been used as biocatalyst, however, analysis of the available sequence space suggests more enzymes to be exploited in synthetic organic chemistry. This review summarizes the current knowledge of this underexplored enzyme class, highlights key molecular, structural, and catalytic features with the aim to provide a comprehensive general description of opine dehydrogenases, thereby supporting future enzyme discovery and protein engineering studies.
意见和类意见化学物质是具有多种生化作用的有价值的天然产物,也是生物活性化合物潜在的合成构建块。它们的合成涉及酮酸与氨基酸的还原胺化反应。这种转化在生产手性纯仲胺方面具有很高的合成潜力。自然界已经进化出用于这种化学的类阿片脱氢酶。迄今为止,虽然只有一种酶被用作生物催化剂,但对可用序列空间的分析表明,还有更多的酶可用于合成有机化学。这篇综述总结了这一未充分探索的酶类的现有知识,强调了关键的分子、结构和催化特征,旨在提供对类阿片脱氢酶的全面综合描述,从而支持未来的酶发现和蛋白质工程研究。