Pipe R K
Histochem J. 1986 Apr;18(4):175-83. doi: 10.1007/BF01676118.
An azo dye technique was used to investigate localization of the acid hydrolase, beta-glucuronidase, at light and electron microscope level in the stomach and digestive gland of the marine periwinkle Littorina littorea. Activity for beta-glucuronidase was located principally within digestive cells of the digestive gland and also associated with the microvillous border and epithelial cells lining the stomach. At the light microscope level all digestive tubules showed activity which appeared essentially restricted to the large heterolysosomes of the digestive cells. However not all digestive cells showed activity. In the electron microscope, reaction product was apparent in all types of macrovesicle in the digestive cells although not all stained positively. Heterophagosomes typically showed reaction product around their periphery or associated with the electron opaque contents. Activity was commonly seen around the apical edge of heterolysosomes where merging of heterophagosomes into heterolysosomes was apparent. Reaction product was commonly located within small electron lucent vesicles which lined the internal membrane of the heterolysosomes but sometimes also associated with flocculent, electron opaque contents. In the stomach dense clusters of reaction product were visible in lysosomes in the basal region of the epithelial cells and in the large granular inclusions of the secretory cells.
采用偶氮染料技术，在光学显微镜和电子显微镜水平上研究了海洋滨螺（Littorina littorea）胃和消化腺中酸性水解酶β-葡萄糖醛酸酶的定位。β-葡萄糖醛酸酶的活性主要位于消化腺的消化细胞内，也与胃内衬的微绒毛边界和上皮细胞有关。在光学显微镜水平上，所有消化小管均显示出活性，且活性基本局限于消化细胞的大型异溶酶体。然而，并非所有消化细胞都显示出活性。在电子显微镜下，尽管并非所有消化细胞都呈阳性染色，但反应产物在消化细胞的所有类型大泡中均很明显。异噬体通常在其周边或与电子不透明内容物相关处显示反应产物。活性常见于异溶酶体的顶端边缘周围，此处异噬体与异溶酶体的融合很明显。反应产物通常位于内衬异溶酶体内膜的小电子透明小泡内，但有时也与絮状、电子不透明内容物相关。在胃中，上皮细胞基部区域的溶酶体以及分泌细胞的大颗粒内含物中可见密集的反应产物簇。
