Pipe R K, Moore M N
Histochem J. 1985 Aug;17(8):939-49. doi: 10.1007/BF01004188.
Azo dye techniques were used to investigate the ultrastructural localization of lysosomal acid hydrolases in ovarian oocytes of the common marine mussel Mytilus edulis. The enzymes were arylsulphatase, beta-glucuronidase, nonspecific esterase, N-acetyl-beta-hexosaminidase and acid phosphatase. For arylsulphatase, the azo dye technique was compared with an alternative method using nitrocatechol sulphate as the substrate and barium as the capturing ion. Activity of all the enzymes was found to be associated with the yolk granules and with pinocytotic phenomena which were observed along the basal membrane of developing oocytes. Activity was also found to be associated with resorption of atretic oocytes.
采用偶氮染料技术研究了常见海洋贻贝紫贻贝卵巢卵母细胞中溶酶体酸性水解酶的超微结构定位。这些酶包括芳基硫酸酯酶、β-葡萄糖醛酸酶、非特异性酯酶、N-乙酰-β-己糖胺酶和酸性磷酸酶。对于芳基硫酸酯酶，将偶氮染料技术与另一种使用硫酸硝基邻苯二酚作为底物和钡作为捕获离子的方法进行了比较。发现所有酶的活性都与卵黄颗粒以及沿发育中卵母细胞基底膜观察到的胞饮现象有关。还发现活性与闭锁卵母细胞的吸收有关。
