莫林通过减少异嗜性细胞细胞外陷阱、氧化应激和炎症反应来保护 T-2 毒素中毒的小鸡。
Morin protects chicks with T-2 toxin poisoning by decreasing heterophil extracellular traps, oxidative stress and inflammatory response.
机构信息
College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province, People's Republic of China.
College of Veterinary Medicine, Southwest University, Chongqing, China.
出版信息
Br Poult Sci. 2023 Oct;64(5):614-624. doi: 10.1080/00071668.2023.2226083. Epub 2023 Jul 7.
- widely exists in a variety of grain feeds. The T-2 toxin is the main hazardous component produced by , making a serious hazard to poultry industry. Morin, belonging to the flavonoid family, can be extracted from mulberry plants and possesses anticancer, antioxidant and anti-inflammatory compounds, but whether morin protects chicks with T-2 toxin poisoning remains unclear. This experiment firstly established a chick model of T-2 toxin poisoning and then investigated the protective effects and mechanism of morin against T-2 toxin in chicks.2. The function of liver and kidney was measured by corresponding alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (Cre) and uric acid (UA) kits. Histopathological changes were observed by haematoxylin-eosin staining. The status of oxidative stress was measured by MDA, SOD, CAT, GSH and GSH-PX kits. The mRNA levels of TNF-α, COX-2, IL-1β, IL-6, caspase-1, caspase-3 and caspase-11 were measured by quantitative real-time PCR. Heterophil extracellular trap (HET) release was analysed by immunofluorescence and fluorescence microplate.3. The model with T-2 toxin poisoning in chicks was successfully established. Morin significantly decreased T-2 toxin-induced ALT, AST, ALP, BUN, Cre and UA, and improved T-2 toxin-induced liver cell rupture, liver cord disorder and kidney interstitial oedema. Oxidative stress analysis showed that morin ameliorated T-2 toxin-induced damage by reducing malondialdehyde (MDA), increasing superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GSH-PX). The qRT-PCR analysis showed that morin reduced T-2 toxin-induced mRNA expressions of TNF-α, COX-2, IL-1β, IL-6, caspase-1, caspase-3 and caspase-11. Moreover, morin significantly reduced the release of T-2 toxin-induced HET and .4. Morin can protect chicks from T-2 toxin poisoning by decreasing HETs, oxidative stress and inflammatory responses, which make it a useful compound against T-2 toxin poisoning in poultry feed.
广泛存在于各种谷物饲料中。T-2 毒素是由镰刀菌属产生的主要有害成分，对家禽业造成严重危害。桑椹中的杨梅素属于黄酮类化合物，具有抗癌、抗氧化和抗炎作用，但杨梅素是否能保护 T-2 毒素中毒的小鸡尚不清楚。本实验首先建立了 T-2 毒素中毒的小鸡模型，然后研究了杨梅素对小鸡 T-2 毒素的保护作用及其机制。
通过相应的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、血尿素氮（BUN）、肌酐（Cre）和尿酸（UA）试剂盒测量肝功能和肾功能。通过苏木精-伊红染色观察组织病理学变化。通过 MDA、SOD、CAT、GSH 和 GSH-PX 试剂盒测量氧化应激状态。通过实时定量 PCR 测量 TNF-α、COX-2、IL-1β、IL-6、caspase-1、caspase-3 和 caspase-11 的 mRNA 水平。通过免疫荧光和荧光微孔板分析嗜中性粒细胞细胞外陷阱（HET）的释放。
成功建立了 T-2 毒素中毒的小鸡模型。杨梅素显著降低了 T-2 毒素诱导的 ALT、AST、ALP、BUN、Cre 和 UA，并改善了 T-2 毒素诱导的肝细胞破裂、肝索紊乱和肾间质水肿。氧化应激分析表明，杨梅素通过降低丙二醛（MDA）、增加超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-PX）来改善 T-2 毒素诱导的损伤。qRT-PCR 分析表明，杨梅素降低了 T-2 毒素诱导的 TNF-α、COX-2、IL-1β、IL-6、caspase-1、caspase-3 和 caspase-11 的 mRNA 表达。此外，杨梅素显著减少了 T-2 毒素诱导的 HET 和的释放。
杨梅素通过减少 HETs、氧化应激和炎症反应来保护小鸡免受 T-2 毒素中毒，这使其成为一种有用的化合物，可用于防治家禽饲料中的 T-2 毒素中毒。
Zotero 插件