College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China.
College of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province 528225, PR China.
Int Immunopharmacol. 2021 Nov;100:108097. doi: 10.1016/j.intimp.2021.108097. Epub 2021 Sep 11.
Zearalenone (ZEA) is a secondary metabolite produced by fungi such as Fusarium and Fusarium flavum, which is classified as a mycotoxin. Crops and feed in a humid surrounding are widely polluted by ZEA, which further endangering the healthful aquaculture of poultry and even human health. Up to now, prevention and cure of mycotoxicosis is still a crucial subject of poultry husbandry. Baicalin (BAI) is a flavonoid refined from dried roots of Scutellaria baicalensis possessing the function of hepatoprotective, anti-inflammatory, anti-oxidant, and anti-atherosclerotic efficacies.etc. But whether Baicalin also has a protective effect against ZEA intoxication is unclear. Therefore, the aim of this study was to establish a model of ZEA-induced toxic injury in chicks, and then to investigate the way in which Baicalin plays a protective role in the mechanism of ZEA-induced liver and kidney injury in chicks. The results exhibit that Baicalin could not only significantly decrease aspartate aminotransferase (AST) , alanine aminotransferase (ALT) and creatinine (Cre) levels in serum, but also ameliorate ZEA-induced pathologic changes of liver and kidney. Baicalin could also significantly regulate ZEA-induced the changes of catalase (CAT) , malondialdehyde (MDA) , total sulfhydryl group , except for glutathione peroxidase (GSH-px) , and inhibit the mRNA levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) , interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) with caspase-3 and caspase-11 in the caspase signaling pathway , meanwhile inhibit the cell apoptosis in immunohistochemistry. In summary, we successfully established a model of ZEA-induced liver injury in chicks, and confirm that Baicalin can reduce ZEA-induced liver and kidney injury in chicks. The mechanism of these effects is via inhibiting inflammation, oxidative stress and apoptosis, which also indicates the potential applicability of Baicalin for the prevention and treatment of ZEA-induced toxicity in chicks.
玉米赤霉烯酮（ZEA）是一种真菌（如镰刀菌和黄色镰刀菌）产生的次生代谢产物，被归类为真菌毒素。在潮湿的环境中，农作物和饲料广泛受到 ZEA 的污染，这进一步危及家禽的健康养殖，甚至人类健康。到目前为止，霉菌毒素中毒的预防和治疗仍然是家禽养殖业的一个关键课题。黄芩苷（BAI）是从黄芩的干根中精制而成的一种黄酮类化合物，具有保肝、抗炎、抗氧化、抗动脉粥样硬化等功效。但是，黄芩苷是否对 ZEA 中毒也具有保护作用尚不清楚。因此，本研究旨在建立 ZEA 诱导的雏鸡毒性损伤模型，然后研究黄芩苷在 ZEA 诱导的雏鸡肝肾功能损伤机制中发挥保护作用的方式。结果表明，黄芩苷不仅能显著降低血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和肌酐（Cre）水平，还能改善 ZEA 诱导的肝肾病变。黄芩苷还能显著调节 ZEA 诱导的过氧化氢酶（CAT）、丙二醛（MDA）、总巯基、除谷胱甘肽过氧化物酶（GSH-px）外的变化，并抑制炎症细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和环氧化酶-2（COX-2）的 mRNA 水平，同时抑制免疫组织化学中的细胞凋亡。总之，我们成功地建立了 ZEA 诱导的雏鸡肝损伤模型，并证实黄芩苷能减轻 ZEA 诱导的雏鸡肝肾功能损伤。这些作用的机制是通过抑制炎症、氧化应激和细胞凋亡，这也表明黄芩苷在预防和治疗 ZEA 诱导的雏鸡毒性方面具有潜在的适用性。
