Department of Cell Biology and Imaging, Jagellonian University, Kraków, Poland.
Exp Brain Res. 2023 Jul;241(7):1887-1904. doi: 10.1007/s00221-023-06649-y. Epub 2023 Jun 19.
A single adenosine receptor gene (dAdoR) has been detected in Drosophila melanogaster. However, its function in different cell types of the nervous system is mostly unknown. Therefore, we overexpressed or silenced the dAdoR gene in eye photoreceptors, all neurons, or glial cells and examined the fitness of flies, the amount and daily pattern of sleep, and the influence of dAdoR silencing on Bruchpilot (BRP) presynaptic protein. Furthermore, we examined the dAdoR and brp gene expression in young and old flies. We found that a higher level of dAdoR in the retina photoreceptors, all neurons, and glial cells negatively influenced the survival rate and lifespan of male and female Drosophila in a cell-dependent manner and to a different extent depending on the age of the flies. In old flies, expression of both dAdoR and brp was higher than in young ones. An excess of dAdoR in neurons improved climbing in older individuals. It also influenced sleep by lengthening nighttime sleep and siesta. In turn, silencing of dAdoR decreased the lifespan of flies, although it increased the survival rate of young flies. It hindered the climbing of older males and females, but did not change sleep. Silencing also affected the daily pattern of BRP abundance, especially when dAdoR expression was decreased in glial cells. The obtained results indicate the role of adenosine and dAdoR in the regulation of fitness in flies that is based on communication between neurons and glial cells, and the effect of glial cells on synapses.
在果蝇中检测到一个单一的腺苷受体基因（dAdoR）。然而，其在神经系统不同细胞类型中的功能大多未知。因此，我们在眼感光细胞、所有神经元或神经胶质细胞中过表达或沉默 dAdoR 基因，并检查果蝇的适应性、睡眠量和日模式，以及沉默 dAdoR 对 Bruchpilot（BRP）突触前蛋白的影响。此外，我们检查了年轻和年老果蝇中的 dAdoR 和 brp 基因表达。我们发现，视网膜感光细胞、所有神经元和神经胶质细胞中 dAdoR 水平的升高以细胞依赖的方式、并根据果蝇的年龄不同程度地对雌雄果蝇的存活率和寿命产生负面影响。在年老的果蝇中，dAdoR 和 brp 的表达均高于年轻的果蝇。神经元中 dAdoR 的过量表达改善了老年个体的攀爬能力。它还通过延长夜间睡眠和午休来影响睡眠。相反，沉默 dAdoR 会降低果蝇的寿命，尽管它会增加年轻果蝇的存活率。它阻碍了年老雄性和雌性的攀爬，但没有改变睡眠。沉默还影响了 BRP 丰度的日模式，尤其是当 dAdoR 在神经胶质细胞中的表达减少时。所得结果表明，腺苷和 dAdoR 在基于神经元和神经胶质细胞之间的通信的果蝇适应性调节中的作用，以及神经胶质细胞对突触的影响。
