Department of Neurosurgery, Beijing Tian tan Hospital, Capital Medical University, Beijing, China.
Department of Intensive Care Unit, Beijing Tian tan Hospital, Capital Medical University, Beijing, China.
Mol Biotechnol. 2024 Jul;66(7):1552-1562. doi: 10.1007/s12033-023-00769-0. Epub 2023 Jun 19.
The central nervous system (CNS) is a complicated neural network. The origin and evolution of functional neurons and glia cells remain unclear, as do the cellular alterations that occur during the course of cerebral disease rehabilitation. Lineage tracing is a valuable method for tracing specific cells and achieving a better understanding of the CNS. Recently, various technological breakthroughs have been made in lineage tracing, such as the application of various combinations of fluorescent reporters and advances in barcode technology. The development of lineage tracing has given us a deeper understanding of the normal physiology of the CNS, especially the pathological processes. In this review, we summarize these advances of lineage tracing and their applications in CNS. We focus on the use of lineage tracing techniques to elucidate the process CNS development and especially the mechanism of injury repair. Deep understanding of the central nervous system will help us to use existing technologies to diagnose and treat diseases.
中枢神经系统（CNS）是一个复杂的神经网络。功能神经元和神经胶质细胞的起源和演化，以及在脑疾病康复过程中发生的细胞变化，仍然不清楚。谱系追踪是一种追踪特定细胞的有价值的方法，可以更好地了解中枢神经系统。最近，谱系追踪技术取得了各种突破，例如各种荧光报告基因组合的应用和条形码技术的进步。谱系追踪的发展使我们对中枢神经系统的正常生理学，特别是病理过程有了更深入的了解。在这篇综述中，我们总结了谱系追踪的这些进展及其在中枢神经系统中的应用。我们重点介绍了使用谱系追踪技术阐明中枢神经系统发育过程，特别是损伤修复机制。对中枢神经系统的深入了解将有助于我们利用现有技术来诊断和治疗疾病。
