Division of Livestock Products Technology, SKUAST-J, Jammu, India.
Division of Animal Biotechnology, SKUAST-K, Srinagar, India.
Ultrason Sonochem. 2023 Aug;98:106482. doi: 10.1016/j.ultsonch.2023.106482. Epub 2023 Jun 15.
Locust protein hydrolysates (LoProHs) pre-processed with microwave and ultrasonication were developed and evaluated for their potential for enhancing the quality of the stored meat emulsion (MEmul). Locust protein (LoPro) samples pre-processed with ultrasonication (Ult) or microwave (Mic) or with no treatment (Not) were hydrolysed with alcalase enzyme (3%). The microwave pre-processed (Mic-LoProHs) and ultrasonicated (Ult-LoProHs) hydrolysates showed significantly (P < 0.05) higher antioxidant [FRAP (ferric reducing antioxidant power) and ABTS and DPPH radical scavenging activities] and antimicrobial [minimum inhibitory concentration (MIC) and inhibitory halos (mm)] potential. The MEmul samples incorporated with Mic-LoProHs and Ult-LoProHs at the maximum level of 1.5% exhibited significantly (P < 0.05) improved results for all the quality parameters such as antioxidant potential (FRAP, ABTS and DPPH), protein oxidation (total carbonyl content), lipid stability, and microbial quality during refrigerated storage (4 ± 1 °C) of two-weeks compared to the control MEmul without any LoProHs. A positive (P < 0.05) impact of the LoProHs was found on the sensory quality of MEmul samples after one week of storage. The digestion simulation improved (P < 0.05) the antioxidant potential of the MEmul samples.
采用微波和超声预处理的蝗虫蛋白水解物(LoProHs)被开发并评估其用于提高储存肉乳液(MEmul)质量的潜力。用超声(Ult)或微波(Mic)预处理或未经处理(Not)的蝗虫蛋白(LoPro)样品用碱性蛋白酶(3%)水解。微波预处理(Mic-LoProHs)和超声处理(Ult-LoProHs)水解物显示出显著(P<0.05)更高的抗氧化[铁还原抗氧化能力(FRAP)和 ABTS 和 DPPH 自由基清除活性]和抗菌[最小抑菌浓度(MIC)和抑菌环(mm)]潜力。在最大水平 1.5%下添加 Mic-LoProHs 和 Ult-LoProHs 的 MEmul 样品在冷藏(4±1°C)两周期间的所有质量参数(如抗氧化潜力(FRAP、ABTS 和 DPPH)、蛋白质氧化(总羰基含量)、脂质稳定性和微生物质量)均显示出显著(P<0.05)的改善,与不含任何 LoProHs 的对照 MEmul 相比。在储存一周后,LoProHs 对 MEmul 样品的感官质量产生了积极的(P<0.05)影响。消化模拟改善(P<0.05)了 MEmul 样品的抗氧化潜力。