Division of Livestock Products Technology, SKUAST-J, Jammu, India.
Division of Animal Biotechnology, SKUAST-K, Srinagar, India.
Ultrason Sonochem. 2023 Aug;98:106482. doi: 10.1016/j.ultsonch.2023.106482. Epub 2023 Jun 15.
Locust protein hydrolysates (LoProHs) pre-processed with microwave and ultrasonication were developed and evaluated for their potential for enhancing the quality of the stored meat emulsion (MEmul). Locust protein (LoPro) samples pre-processed with ultrasonication (Ult) or microwave (Mic) or with no treatment (Not) were hydrolysed with alcalase enzyme (3%). The microwave pre-processed (Mic-LoProHs) and ultrasonicated (Ult-LoProHs) hydrolysates showed significantly (P < 0.05) higher antioxidant [FRAP (ferric reducing antioxidant power) and ABTS and DPPH radical scavenging activities] and antimicrobial [minimum inhibitory concentration (MIC) and inhibitory halos (mm)] potential. The MEmul samples incorporated with Mic-LoProHs and Ult-LoProHs at the maximum level of 1.5% exhibited significantly (P < 0.05) improved results for all the quality parameters such as antioxidant potential (FRAP, ABTS and DPPH), protein oxidation (total carbonyl content), lipid stability, and microbial quality during refrigerated storage (4 ± 1 °C) of two-weeks compared to the control MEmul without any LoProHs. A positive (P < 0.05) impact of the LoProHs was found on the sensory quality of MEmul samples after one week of storage. The digestion simulation improved (P < 0.05) the antioxidant potential of the MEmul samples.
采用微波和超声预处理的蝗虫蛋白水解物（LoProHs）被开发并评估其用于提高储存肉乳液（MEmul）质量的潜力。用超声（Ult）或微波（Mic）预处理或未经处理（Not）的蝗虫蛋白（LoPro）样品用碱性蛋白酶（3%）水解。微波预处理（Mic-LoProHs）和超声处理（Ult-LoProHs）水解物显示出显著（P<0.05）更高的抗氧化[铁还原抗氧化能力（FRAP）和 ABTS 和 DPPH 自由基清除活性]和抗菌[最小抑菌浓度（MIC）和抑菌环（mm）]潜力。在最大水平 1.5%下添加 Mic-LoProHs 和 Ult-LoProHs 的 MEmul 样品在冷藏（4±1°C）两周期间的所有质量参数（如抗氧化潜力（FRAP、ABTS 和 DPPH）、蛋白质氧化（总羰基含量）、脂质稳定性和微生物质量）均显示出显著（P<0.05）的改善，与不含任何 LoProHs 的对照 MEmul 相比。在储存一周后，LoProHs 对 MEmul 样品的感官质量产生了积极的（P<0.05）影响。消化模拟改善（P<0.05）了 MEmul 样品的抗氧化潜力。
