基于金纳米颗粒的侧向流动免疫分析快速灵敏检测葡萄、番茄和黄瓜样品中的唑虫酰胺。
Lateral flow immunoassay based on gold nanoparticles for rapid and sensitive detection of zoxamide in grape, tomato and cucumber samples.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
出版信息
Food Chem. 2023 Nov 15;426:136533. doi: 10.1016/j.foodchem.2023.136533. Epub 2023 Jun 7.
In the study, we discovered zoxamide hapten (ZOX-hapten) by introducing a carboxyl extension chain, combined it with protein to make a complete antigen to immunize mice, and generated a monoclonal antibody (mAb) against ZOX. To identify ZOX residues in grape, tomato, and cucumber samples, we used our anti-ZOX mAb to develop a lateral flow immunoassay (LFIA) strip. In grape, tomato, and cucumber samples, the calculated detection limit of the LFIA strip in grape, tomato and cucumber samples was 3.44, 4.78 and 3.53 ng/g, respectively. Using the LFIA strip, the recovery rate from grape samples was 96.4-106.8%, and that from tomato samples was 98.4-107.5%, while the recovery from cucumber samples was 99.4-111.3%. These results showed that our LFIA strip could be expected to achieve rapid screening of ZOX residues in fruits and vegetables.
在这项研究中，我们通过引入羧基延伸链发现了唑虫酰胺半抗原（ZOX-hapten），将其与蛋白质结合制成完整抗原免疫小鼠，生成了针对 ZOX 的单克隆抗体（mAb）。为了鉴定葡萄、番茄和黄瓜样品中的 ZOX 残留，我们使用抗 ZOX mAb 开发了侧向流动免疫分析（LFIA）试纸条。在葡萄、番茄和黄瓜样品中，LFIA 试纸条在葡萄、番茄和黄瓜样品中的计算检测限分别为 3.44、4.78 和 3.53ng/g。使用 LFIA 试纸条，从葡萄样品中的回收率为 96.4-106.8%，从番茄样品中的回收率为 98.4-107.5%，而从黄瓜样品中的回收率为 99.4-111.3%。这些结果表明，我们的 LFIA 试纸条有望实现水果和蔬菜中 ZOX 残留的快速筛选。
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