International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
Analyst. 2022 Aug 8;147(16):3684-3691. doi: 10.1039/d2an00899h.
Residues of flumetralin, a dinitroaniline herbicide, in foods pose a health risk to humans. In this study, we produced a sensitive monoclonal antibody (mAb) against flumetralin and developed a gold nanoparticle-based lateral flow immunoassay (LFIA) strip for screening flumetralin in orange. The subtype of mAb 3F5 was IgG1 and had a half-maximal inhibitory concentration of 9.43 ng mL. The cross-reactivity assay indicated that mAb had good specificity. The LFIA strip had a visual limit of detection of 200 ng g in orange. We obtained results with the naked eye within 15 min. The calculated LOD of the strip in orange was 33.26 ng g, with a linear detection of 80.72-1672.10 ng g. The strip had recovery rates ranging from 98.4% to 110.1%. Therefore, our developed method is an effective and reliable tool for the rapid detection of flumetralin in orange.
氟节胺是一种二硝基苯胺类除草剂，其在食物中的残留会对人类健康构成威胁。本研究制备了针对氟节胺的高灵敏单克隆抗体，并开发了一种基于金纳米颗粒的胶体金侧向流动免疫检测试纸条，用于快速筛选橙汁中的氟节胺残留。该单克隆抗体 3F5 的亚型为 IgG1，半数抑制浓度为 9.43ng/mL。交叉反应实验表明，该单克隆抗体具有良好的特异性。胶体金侧向流动免疫检测试纸条对橙汁中氟节胺的检测限为 200ng/g，肉眼检测结果在 15min 内即可获得。该方法在橙汁中的检出限为 33.26ng/g，线性检测范围为 80.72-1672.10ng/g。该方法的回收率为 98.4%-110.1%。因此，本研究建立的方法可用于快速检测橙汁中的氟节胺残留。
