不同直径的非功能化聚苯乙烯纳米颗粒对人红细胞膜和形态的影响。

The effects of non-functionalized polystyrene nanoparticles with different diameters on human erythrocyte membrane and morphology.

机构信息

Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska Str. 141/143, 90-236 Lodz, Poland.

Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.

出版信息

Toxicol In Vitro. 2023 Sep;91:105634. doi: 10.1016/j.tiv.2023.105634. Epub 2023 Jun 17.

DOI:10.1016/j.tiv.2023.105634

PMID:37336462

Abstract

In this study, the potential toxicity of non-functionalized polystyrene nanoparticles (PS-NPs) in human erythrocytes has been assessed. The effect of PS-NPs with different diameters (∼30 nm, ∼45 nm, ∼70 nm) on fluidity of erythrocytes membrane, red blood cells shape, as well as haemolysis of these cells has been investigated. Erythrocytes were incubated for 24 h with non-functionalized PS-NPs in concentrations ranging from 0.001 to 200 μg/mL in order to study haemolysis and from 0.001 to 10 μg/mL to determine other parameters. Fluidity was estimated by electron paramagnetic resonance (EPR) and the fluorimetric method. It has been shown that PS-NPs induced haemolysis, caused changes in the fluidity of red blood cells membrane, and altered their shape. Non-functionalized PS-NPs increased the membrane stiffness in the hydrophobic region of hydrocarbon chains of fatty acids. The observed changes in haemolysis and morphology were dependent on the size of the nanoparticles. The smallest PS-NPs of ∼30 nm (with the smallest absolute value of the negative zeta potential -29.68 mV) induced the greatest haemolysis, while the largest PS-NPs of ∼70 nm (with the highest absolute value of the negative zeta potential -42.00 mV) caused the greatest changes in erythrocyte shape and stomatocytes formation.

摘要

在这项研究中，评估了非功能化聚苯乙烯纳米粒子（PS-NPs）对人红细胞的潜在毒性。研究了不同直径（约 30nm、约 45nm、约 70nm）的 PS-NPs 对红细胞膜流动性、红细胞形状以及这些细胞溶血的影响。将红细胞与非功能化 PS-NPs 在浓度为 0.001 至 200μg/mL 的范围内孵育 24 小时，以研究溶血，并在 0.001 至 10μg/mL 的范围内测定其他参数。通过电子顺磁共振（EPR）和荧光法估计流动性。结果表明，PS-NPs 诱导溶血，导致红细胞膜流动性发生变化，并改变其形状。非功能化 PS-NPs 增加了脂肪酸烃链疏水区域的膜硬度。溶血和形态的观察到的变化取决于纳米粒子的大小。最小的 PS-NPs（约 30nm，其负 zeta 电位绝对值最小为-29.68mV）诱导最大的溶血，而最大的 PS-NPs（约 70nm，其负 zeta 电位绝对值最大为-42.00mV）导致红细胞形状最大变化和形成棘细胞。