Shandong Food Ferment Industry Research & Design Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250013, China.
School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Lett Appl Microbiol. 2023 Jul 3;76(7). doi: 10.1093/lambio/ovad073.
One thermotolerant Bacillus strain SFLV-ZM107 which could produce large amounts of extracellular polysaccharides (EPS) at 50°C was identified as Bacillus licheniformis by 16S rDNA sequencing and physiological and biochemical experiments. The EPS was identified as levan fructan by monosaccharides determination, NMR, and FTIR. Strain SFLV-ZM107 can be grown in the range of 35-55°C and it is efficient to produce levan from 400 to 500 g l-1 of sucrose. The production of levan can reach 158 g l-1 in a 5 L tank fermentation with an initial sucrose concentration of 450 g l-1 at 50°C for 12 hours with a maximum productivity of 13.17 g l-1 h-1. To the best of our knowledge, the strains obtained in this study are the most productive and efficient, which has great prospects for industrial application.
一株能够在 50°C 下大量产生胞外多糖（EPS）的耐热芽孢杆菌 SFLV-ZM107 通过 16S rDNA 测序和生理生化实验被鉴定为地衣芽孢杆菌。通过单糖测定、NMR 和 FTIR 鉴定，该 EPS 为蔗果三糖。菌株 SFLV-ZM107 可在 35-55°C 范围内生长，从 400 至 500 g/L 的蔗糖中高效生产蔗果三糖。在 50°C 下初始蔗糖浓度为 450 g/L 的 5 L 发酵罐中发酵 12 小时，可达到 158 g/L 的蔗果三糖产量，最大比生产速率为 13.17 g/L·h-1。据我们所知，本研究获得的菌株是产率和效率最高的，具有广阔的工业应用前景。
