虫荧光素甲酯可照亮多种丝氨酸水解酶的活性。
Firefly luciferin methyl ester illuminates the activity of multiple serine hydrolases.
机构信息
Department of Biochemistry and Molecular Biotechnology, UMass Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA.
出版信息
Chem Commun (Camb). 2023 Jul 6;59(55):8552-8555. doi: 10.1039/d3cc02540c.
Abstract
Firefly luciferin methyl ester is hydrolyzed by monoacylglycerol lipase MAGL, amidase FAAH, poorly-characterized hydrolase ABHD11, and hydrolases known for -depalmitoylation (LYPLA1/2), not just esterase CES1. This enables activity-based bioluminescent assays for serine hydrolases and suggests that the 'esterase activity' responsible for hydrolyzing ester prodrugs is more diverse than previously supposed.
摘要
萤火虫荧光素甲酯被单酰基甘油脂肪酶 MAGL、酰胺酶 FAAH、特征不明确的水解酶 ABHD11 以及已知具有去棕榈酰化作用的水解酶（LYPLA1/2）水解，而不仅仅是酯酶 CES1。这使得基于活性的丝氨酸水解酶生物发光测定法成为可能，并表明负责水解酯前药的“酯酶活性”比以前想象的更为多样化。
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