Department of Chemistry, University of California, Irvine, Irvine, California 92697, United States.
Chemical Biology Laboratory, Cancer for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, United States.
J Am Chem Soc. 2023 Feb 15;145(6):3335-3345. doi: 10.1021/jacs.2c07220. Epub 2023 Feb 6.
Multicomponent bioluminescence imaging requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging . The CouLuc-3 scaffolds are also orthogonal to popular bioluminescent reporters and can be used for multicomponent imaging applications. Collectively, this work showcases a new set of bioluminescent tools that can be readily implemented for multiplexed imaging in a variety of biological settings.
多分量生物发光成像是需要扩展组织穿透探针的集合。为此，我们开发了一类新型的近红外（NIR）发射香豆素荧光素类似物（CouLuc-3s）。这些支架可以从商业上可用的染料中轻松获得。还鉴定了用于 CouLuc-3 类似物的互补突变荧光素酶。最亮的探针可实现灵敏的成像。CouLuc-3 支架还与流行的生物发光报告基因正交，可以用于多分量成像应用。总的来说，这项工作展示了一组新的生物发光工具，可以很容易地在各种生物环境中实现多重成像。
